Embryonic environmental niche reprograms somatic cells to express pluripotency markers and participate in adult chimaeras
Modliński, J. A.
AffiliationDepartment of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec,05-552 Magdalenka, Poland.
MetadataShow full item record
AbstractThe phenomenon of the reprogramming of terminally differentiated cells can be achieved by various means, like somatic cell nuclear transfer, cell fusion with a pluripotent cell, or the introduction of pluripotency genes. Here, we present the evidence that somatic cells can attain the expression of pluripotency markers after their introduction into early embryos. Mouse embryonic fibroblasts introduced between blastomeres of cleaving embryos, within two days of in vitro culture, express transcription factors specific to blastocyst lineages, including pluripotency factors. Analysis of donor tissue marker DNA has revealed that the progeny of introduced cells are found in somatic tissues of foetuses and adult chimaeras, providing evidence for cell reprogramming. Analysis of ploidy has shown that in the chimaeras, the progeny of introduced cells are either diploid or tetraploid, the latter indicating cell fusion. The presence of donor DNA in diploid cells from chimaeric embryos proved that the non-fused progeny of introduced fibroblasts persisted in chimaeras, which is evidence of reprogramming by embryonic niche. When adult somatic (cumulus) cells were introduced into early cleavage embryos, the extent of integration was limited and only cell fusion-mediated reprogramming was observed. These results show that both cell fusion and cell interactions with the embryonic niche reprogrammed somatic cells towards pluripotency.
CitationZyzynska-Galenska K, Bernat A, Piliszek A, Karasiewicz J, Szablisty E, Sacharczuk M, et al. Embryonic Environmental Niche Reprograms Somatic Cells to Express Pluripotency Markers and Participate in Adult Chimaeras. Cells. 2021;10(3).
- Reprogramming of two somatic nuclei in the same ooplasm leads to pluripotent embryonic stem cells.
- Authors: Pfeiffer MJ, Esteves TC, Balbach ST, Araúzo-Bravo MJ, Stehling M, Jauch A, Houghton FD, Schwarzer C, Boiani M
- Issue date: 2013 Nov
- Cell fusion for reprogramming pluripotency: toward elimination of the pluripotent genome.
- Authors: Pralong D, Trounson AO, Verma PJ
- Issue date: 2006
- The H3K27 demethylase Utx regulates somatic and germ cell epigenetic reprogramming.
- Authors: Mansour AA, Gafni O, Weinberger L, Zviran A, Ayyash M, Rais Y, Krupalnik V, Zerbib M, Amann-Zalcenstein D, Maza I, Geula S, Viukov S, Holtzman L, Pribluda A, Canaani E, Horn-Saban S, Amit I, Novershtern N, Hanna JH
- Issue date: 2012 Aug 16
- Reprogramming of Extraembryonic Trophoblast Stem Cells into Embryonic Pluripotent State by Fusion with Embryonic Stem Cells.
- Authors: Hong YJ, Hong K, Byun S, Choi HW, Do JT
- Issue date: 2018 Oct 1
- Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells.
- Authors: Ambrosi DJ, Tanasijevic B, Kaur A, Obergfell C, O'Neill RJ, Krueger W, Rasmussen TP
- Issue date: 2007 May