Embryonic environmental niche reprograms somatic cells to express pluripotency markers and participate in adult chimaeras
Authors
Żyżyńska-Galeńska, K.Bernat, A.
Piliszek, A.
Karasiewicz, J.
Szablisty, E.
Sacharczuk, M.
Brewińska-Olchowik, M.
Bochenek, M.
Grabarek, Joanna
Modliński, J. A.
Affiliation
Department of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec,05-552 Magdalenka, Poland.Issue Date
2021
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The phenomenon of the reprogramming of terminally differentiated cells can be achieved by various means, like somatic cell nuclear transfer, cell fusion with a pluripotent cell, or the introduction of pluripotency genes. Here, we present the evidence that somatic cells can attain the expression of pluripotency markers after their introduction into early embryos. Mouse embryonic fibroblasts introduced between blastomeres of cleaving embryos, within two days of in vitro culture, express transcription factors specific to blastocyst lineages, including pluripotency factors. Analysis of donor tissue marker DNA has revealed that the progeny of introduced cells are found in somatic tissues of foetuses and adult chimaeras, providing evidence for cell reprogramming. Analysis of ploidy has shown that in the chimaeras, the progeny of introduced cells are either diploid or tetraploid, the latter indicating cell fusion. The presence of donor DNA in diploid cells from chimaeric embryos proved that the non-fused progeny of introduced fibroblasts persisted in chimaeras, which is evidence of reprogramming by embryonic niche. When adult somatic (cumulus) cells were introduced into early cleavage embryos, the extent of integration was limited and only cell fusion-mediated reprogramming was observed. These results show that both cell fusion and cell interactions with the embryonic niche reprogrammed somatic cells towards pluripotency.Citation
Zyzynska-Galenska K, Bernat A, Piliszek A, Karasiewicz J, Szablisty E, Sacharczuk M, et al. Embryonic Environmental Niche Reprograms Somatic Cells to Express Pluripotency Markers and Participate in Adult Chimaeras. Cells. 2021;10(3).Journal
CellsDOI
10.3390/cells10030490PubMed ID
33668852Additional Links
https://dx.doi.org/10.3390/cells10030490Type
ArticleLanguage
enae974a485f413a2113503eed53cd6c53
10.3390/cells10030490
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