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    Embryonic environmental niche reprograms somatic cells to express pluripotency markers and participate in adult chimaeras

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    Authors
    Żyżyńska-Galeńska, K.
    Bernat, A.
    Piliszek, A.
    Karasiewicz, J.
    Szablisty, E.
    Sacharczuk, M.
    Brewińska-Olchowik, M.
    Bochenek, M.
    Grabarek, Joanna
    Modliński, J. A.
    Affiliation
    Department of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec,05-552 Magdalenka, Poland.
    Issue Date
    2021
    
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    Abstract
    The phenomenon of the reprogramming of terminally differentiated cells can be achieved by various means, like somatic cell nuclear transfer, cell fusion with a pluripotent cell, or the introduction of pluripotency genes. Here, we present the evidence that somatic cells can attain the expression of pluripotency markers after their introduction into early embryos. Mouse embryonic fibroblasts introduced between blastomeres of cleaving embryos, within two days of in vitro culture, express transcription factors specific to blastocyst lineages, including pluripotency factors. Analysis of donor tissue marker DNA has revealed that the progeny of introduced cells are found in somatic tissues of foetuses and adult chimaeras, providing evidence for cell reprogramming. Analysis of ploidy has shown that in the chimaeras, the progeny of introduced cells are either diploid or tetraploid, the latter indicating cell fusion. The presence of donor DNA in diploid cells from chimaeric embryos proved that the non-fused progeny of introduced fibroblasts persisted in chimaeras, which is evidence of reprogramming by embryonic niche. When adult somatic (cumulus) cells were introduced into early cleavage embryos, the extent of integration was limited and only cell fusion-mediated reprogramming was observed. These results show that both cell fusion and cell interactions with the embryonic niche reprogrammed somatic cells towards pluripotency.
    Citation
    Zyzynska-Galenska K, Bernat A, Piliszek A, Karasiewicz J, Szablisty E, Sacharczuk M, et al. Embryonic Environmental Niche Reprograms Somatic Cells to Express Pluripotency Markers and Participate in Adult Chimaeras. Cells. 2021;10(3).
    Journal
    Cells
    URI
    http://hdl.handle.net/10541/623892
    DOI
    10.3390/cells10030490
    PubMed ID
    33668852
    Additional Links
    https://dx.doi.org/10.3390/cells10030490
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.3390/cells10030490
    Scopus Count
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    All Paterson Institute for Cancer Research

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