• Login
    View Item 
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Developing tumor radiosensitivity signatures using LncRNAs

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Khan, Mairah T
    Yang, Lingjian
    More, Elisabet
    Irlam-Jones, Joely J
    Valentine, Helen R
    Hoskin, Peter J
    Choudhury, Ananya
    West, Catharine M L
    Affiliation
    Translational Radiobiology Group, Division of Cancer Sciences, University of Manchester, Manchester Academic Health Science Centre, Christie NHS Foundation Trust Hospital, Manchester M20 4BX
    Issue Date
    2021
    
    Metadata
    Show full item record
    Abstract
    Long non-coding RNAs (lncRNAs) are involved in diverse biological processes, including DNA damage repair, and are of interest as potential biomarkers of radiosensitivity. We investigated whether lncRNA radiosensitivity signatures could be derived for use in cancer patients treated with radiotherapy. Signature development involved radiosensitivity measurements for cell lines and primary tumor samples, and patient outcome after radiotherapy. A 10-lncRNA signature trained on radiosensitivity measurements in bladder cell lines showed a trend towards independent validation. In multivariable analyses, patients with tumors classified as radioresistant by the lncRNA signature had poorer local relapse-free survival (P = 0.065) in 151 patients with muscle-invasive bladder cancer who underwent radiotherapy. An mRNA-based radiosensitivity index signature performed similarly to the lncRNA bladder signature for local relapse-free survival (P = 0.055). Pathway analysis showed the lncRNA signature associated with molecular processes involved in radiation responses. Knockdown of one of the lncRNAs in the signature showed a modest increase in radiosensitivity in one cell line. An alternative approach involved training on primary cervical tumor radiosensitivity or local control after radiotherapy. Both approaches failed to generate a cervix lncRNA radiosensitivity signature, which was attributed to the age of samples in our cohorts. Our work highlights challenges in validating lncRNA signatures as biomarkers in archival tissue from radiotherapy cohorts, but supports continued investigation of lncRNAs for a role in radiosensitivity.
    Citation
    Khan MT, Yang L, More E, Irlam-Jones JJ, Valentine HR, Hoskin P, et al. Developing Tumor Radiosensitivity Signatures Using LncRNAs. Radiat Res. 2021.
    Journal
    Radiation Research
    URI
    http://hdl.handle.net/10541/623885
    DOI
    10.1667/rade-20-00157.1
    PubMed ID
    33577642
    Additional Links
    https://dx.doi.org/10.1667/rade-20-00157.1
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.1667/rade-20-00157.1
    Scopus Count
    Collections
    All Christie Publications

    entitlement

    Related articles

    • A three-lncRNA signature predicts overall survival and disease-free survival in patients with esophageal squamous cell carcinoma.
    • Authors: Huang GW, Xue YJ, Wu ZY, Xu XE, Wu JY, Cao HH, Zhu Y, He JZ, Li CQ, Li EM, Xu LY
    • Issue date: 2018 Feb 6
    • Long Noncoding RNA FAM201A Mediates the Radiosensitivity of Esophageal Squamous Cell Cancer by Regulating ATM and mTOR Expression via miR-101.
    • Authors: Chen M, Liu P, Chen Y, Chen Z, Shen M, Liu X, Li X, Li A, Lin Y, Yang R, Ni W, Zhou X, Zhang L, Tian Y, Li J, Chen J
    • Issue date: 2018
    • A prognostic index based on a fourteen long non-coding RNA signature to predict the recurrence-free survival for muscle-invasive bladder cancer patients.
    • Authors: Zhang X, Zhang M, Zhang X, Zhu X, Wang J
    • Issue date: 2020 Jul 9
    • A three-lncRNA signature predicts clinical outcomes in low-grade glioma patients after radiotherapy.
    • Authors: Lin W, Huang Z, Xu Y, Chen X, Chen T, Ye Y, Ding J, Chen Z, Chen L, Qiu X, Qiu S
    • Issue date: 2020 May 26
    • Six-long non-coding RNA signature predicts recurrence-free survival in hepatocellular carcinoma.
    • Authors: Gu JX, Zhang X, Miao RC, Xiang XH, Fu YN, Zhang JY, Liu C, Qu K
    • Issue date: 2019 Jan 14
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.