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    Antitumor effects of self-assembling peptide-emodin in situ hydrogels in vitro and in vivo

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    Authors
    Wei, W.
    Tang, Jianhua
    Li, H.
    Huang, Y.
    Yin, C.
    Li, D.
    Tang, F.
    Affiliation
    Department of Clinical Pharmacy, Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi 563000, People's Republic of China.
    Issue Date
    2021
    
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    Abstract
    Purpose: To study the in vitro and in vivo antitumor effects of the colloidal suspension-in situ hydrogel of emodin (EM) constructed with the self-assembling peptide RADA16-I and systematically evaluate the feasibility of the delivery system. Methods: The MTT and colony-formation assays were used to determine the viability of normal cells NCTC 1469 and tumor cells Hepa1-6. The uptake of EM in the RADA16-I-EM in situ hydrogel by tumor cells was analyzed by laser confocal microscope and flow cytometry. Flow cytometry was used to detect the cell apoptosis and cell cycle distribution. Transwell assay was used to detect the migration and invasion of tumor cells. The antitumor efficacy of the RADA16-I-EM in situ hydrogel and its toxic effects was further assessed in vivo on Hepa1-6 tumor-bearing C57 mice. Results: The results showed that the RADA16-I-EM in situ hydrogels could obviously reduce the toxicity of EM to normal cells and the survival of tumor cells. The uptake of EM by the cells from the hydrogels was obviously increased and could significantly induce apoptosis and arrest cell cycle in the G2/M phase, and reduce the migration, invasion and clone-formation ability of the cells. The RADA16-I-EM in situ hydrogel could also effectively inhibit the tumor growth and obviously decrease the toxic effects of EM on normal tissues in vivo. Conclusion: Our results demonstrated that RADA16-I has the potential to be a carrier for the hydrophobic drug EM and can effectively improve the delivery of hydrophobic antitumor drugs with enhanced antitumor effects and reduced toxic effects of the drugs on normal cells and tissues.
    Citation
    Wei W, Tang J, Li H, Huang Y, Yin C, Li D, et al. Antitumor Effects of Self-Assembling Peptide-Emodin in situ Hydrogels in vitro and in vivo. Int J Nanomedicine. 2021;16:47-60.
    Journal
    International Journal of Nanomedicine
    URI
    http://hdl.handle.net/10541/623809
    DOI
    10.2147/ijn.S282154
    PubMed ID
    33442249
    Additional Links
    https://dx.doi.org/10.2147/ijn.S282154
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.2147/ijn.S282154
    Scopus Count
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    All Paterson Institute for Cancer Research

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