A practical guide to cancer subclonal reconstruction from DNA sequencing
Authors
Tarabichi, M.Salcedo, A.
Deshwar, A. G.
Ni Leathlobhair, M.
Wintersinger, J.
Wedge, David C
Van Loo, P.
Morris, Q. D.
Boutros, P. C.
Affiliation
The Francis Crick Institute, London, UK.Issue Date
2021
Metadata
Show full item recordAbstract
Subclonal reconstruction from bulk tumor DNA sequencing has become a pillar of cancer evolution studies, providing insight into the clonality and relative ordering of mutations and mutational processes. We provide an outline of the complex computational approaches used for subclonal reconstruction from single and multiple tumor samples. We identify the underlying assumptions and uncertainties in each step and suggest best practices for analysis and quality assessment. This guide provides a pragmatic resource for the growing user community of subclonal reconstruction methods.Citation
Tarabichi M, Salcedo A, Deshwar AG, Ni Leathlobhair M, Wintersinger J, Wedge DC, et al. A practical guide to cancer subclonal reconstruction from DNA sequencing. Nat Methods. 2021.Journal
Nature MethodsDOI
10.1038/s41592-020-01013-2PubMed ID
33398189Additional Links
https://dx.doi.org/10.1038/s41592-020-01013-2Type
ArticleLanguage
enae974a485f413a2113503eed53cd6c53
10.1038/s41592-020-01013-2
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