AffiliationManchester Fungal Infection Group, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, Core Technology Facility, The University of Manchester, Manchester, UK.
MetadataShow full item record
AbstractCRISPR/Cas9 technology enables rapid and efficient genome editing in a variety of experimental systems. Genome editing using CRISPR/Cas9 has become an increasingly popular genetic engineering tool due to (1) an extensive array of commercial ready-to-use CRIPSR/Cas9 systems, (2) improved efficiency of cell delivery, and (3) the possibility to do multigene editing. Here, we describe a method to introduce single gene disruption in lung bronchial epithelial cells. This approach can be used to study host factors important for pathogen interaction or to identify and study genetic markers determining susceptibility to fungal disease.
CitationGago S, Overton NLD, Bowyer P. CRISPR-Cas9-Mediated Gene Silencing in Cultured Human Epithelia. Methods Mol Biol. 2021;2260:37-47.
JournalMethods in Molecular Biology
- Gene Therapy with CRISPR/Cas9 Coming to Age for HIV Cure.
- Authors: Soriano V
- Issue date: 2017 Oct-Dec
- CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective.
- Authors: Song R, Zhai Q, Sun L, Huang E, Zhang Y, Zhu Y, Guo Q, Tian Y, Zhao B, Lu H
- Issue date: 2019 Sep
- Two Distinct Approaches for CRISPR-Cas9-Mediated Gene Editing in Cryptococcus neoformans and Related Species.
- Authors: Wang P
- Issue date: 2018 Jun 27
- Two CRISPR/Cas9 Systems Developed in Thermomyces dupontii and Characterization of Key Gene Functions in Thermolide Biosynthesis and Fungal Adaptation.
- Authors: Huang WP, Du YJ, Yang Y, He JN, Lei Q, Yang XY, Zhang KQ, Niu XM
- Issue date: 2020 Oct 1
- Forced Recycling of an AMA1-Based Genome-Editing Plasmid Allows for Efficient Multiple Gene Deletion/Integration in the Industrial Filamentous Fungus <i>Aspergillus oryzae</i>.
- Authors: Katayama T, Nakamura H, Zhang Y, Pascal A, Fujii W, Maruyama JI
- Issue date: 2019 Feb 1