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    CRISPR-Cas9-mediated gene silencing in cultured human epithelia

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    Authors
    Gago, S.
    Overton, Nicola L D
    Bowyer, P.
    Affiliation
    Manchester Fungal Infection Group, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, Core Technology Facility, The University of Manchester, Manchester, UK.
    Issue Date
    2021
    
    Metadata
    Show full item record
    Abstract
    CRISPR/Cas9 technology enables rapid and efficient genome editing in a variety of experimental systems. Genome editing using CRISPR/Cas9 has become an increasingly popular genetic engineering tool due to (1) an extensive array of commercial ready-to-use CRIPSR/Cas9 systems, (2) improved efficiency of cell delivery, and (3) the possibility to do multigene editing. Here, we describe a method to introduce single gene disruption in lung bronchial epithelial cells. This approach can be used to study host factors important for pathogen interaction or to identify and study genetic markers determining susceptibility to fungal disease.
    Citation
    Gago S, Overton NLD, Bowyer P. CRISPR-Cas9-Mediated Gene Silencing in Cultured Human Epithelia. Methods Mol Biol. 2021;2260:37-47.
    Journal
    Methods in Molecular Biology
    URI
    http://hdl.handle.net/10541/623739
    DOI
    10.1007/978-1-0716-1182-1_4
    PubMed ID
    33405030
    Additional Links
    https://dx.doi.org/10.1007/978-1-0716-1182-1_4
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.1007/978-1-0716-1182-1_4
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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