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dc.contributor.authorPedley, R.
dc.contributor.authorKing, L. E.
dc.contributor.authorMallikarjun, V.
dc.contributor.authorWang, Pengbo
dc.contributor.authorSwift, J.
dc.contributor.authorBrennan, K.
dc.contributor.authorGilmore, A. P.
dc.date.accessioned2020-11-16T07:49:15Z
dc.date.available2020-11-16T07:49:15Z
dc.date.issued2020en
dc.identifier.citationPedley R, King LE, Mallikarjun V, Wang P, Swift J, Brennan K, et al. BioID-based proteomic analysis of the Bid interactome identifies novel proteins involved in cell-cycle-dependent apoptotic priming. Cell Death Dis. 2020;11(10):872.en
dc.identifier.pmid33067418en
dc.identifier.doi10.1038/s41419-020-03091-8en
dc.identifier.urihttp://hdl.handle.net/10541/623424
dc.description.abstractApoptotic priming controls the commitment of cells to apoptosis by determining how close they lie to mitochondrial permeabilisation. Variations in priming are important for how both healthy and cancer cells respond to chemotherapeutic agents, but how it is dynamically coordinated by Bcl-2 proteins remains unclear. The Bcl-2 family protein Bid is phosphorylated when cells enter mitosis, increasing apoptotic priming and sensitivity to antimitotic drugs. Here, we report an unbiased proximity biotinylation (BioID) screen to identify regulators of apoptotic priming in mitosis, using Bid as bait. The screen primarily identified proteins outside of the canonical Bid interactome. Specifically, we found that voltage-dependent anion-selective channel protein 2 (VDAC2) was required for Bid phosphorylation-dependent changes in apoptotic priming during mitosis. These results highlight the importance of the wider Bcl-2 family interactome in regulating the temporal control of apoptotic priming.en
dc.titleBioID-based proteomic analysis of the Bid interactome identifies novel proteins involved in cell-cycle-dependent apoptotic primingen
dc.typeArticleen
dc.contributor.departmentWellcome Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, UK.en
dc.identifier.journalCell Death and Diseaseen
dc.description.noteen]
refterms.dateFOA2020-11-17T09:17:50Z


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