Show simple item record

dc.contributor.authorGonzález-Prieto, R.
dc.contributor.authorCabello-Lobato, Maria J
dc.contributor.authorPrado, F.
dc.date.accessioned2020-10-06T13:33:49Z
dc.date.available2020-10-06T13:33:49Z
dc.date.issued2021en
dc.identifier.citationGonzalez-Prieto R, Cabello-Lobato MJ, Prado F. In Vivo Binding of Recombination Proteins to Non-DSB DNA Lesions and to Replication Forks. Methods Mol Biol. 2021;2153:447-58.en
dc.identifier.pmid32840798en
dc.identifier.doi10.1007/978-1-0716-0644-5_31en
dc.identifier.urihttp://hdl.handle.net/10541/623347
dc.description.abstractHomologous recombination (HR) has been extensively studied in response to DNA double-strand breaks (DSBs). In contrast, much less is known about how HR deals with DNA lesions other than DSBs (e.g., at single-stranded DNA) and replication forks, despite the fact that these DNA structures are associated with most spontaneous recombination events. A major handicap for studying the role of HR at non-DSB DNA lesions and replication forks is the difficulty of discriminating whether a recombination protein is associated with the non-DSB lesion per se or rather with a DSB generated during their processing. Here, we describe a method to follow the in vivo binding of recombination proteins to non-DSB DNA lesions and replication forks. This approach is based on the cleavage and subsequent electrophoretic analysis of the target DNA by the recombination protein fused to the micrococcal nuclease.en
dc.language.isoenen
dc.relation.urlhttps://dx.doi.org/10.1007/978-1-0716-0644-5_31en
dc.titleIn vivo binding of recombination proteins to non-DSB DNA lesions and to replication forksen
dc.typeArticleen
dc.contributor.departmentDepartment of Cell and Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands.en
dc.identifier.journalMethods in Molecular Biologyen
dc.description.noteen]


This item appears in the following Collection(s)

Show simple item record