Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability
Authors
Prendergast, LisaMcClurg, U. L.
Hristova, R.
Berlinguer-Palmini, R.
Greener, S.
Veitch, K.
Hernandez, I.
Pasero, P.
Rico, D.
Higgins, J. M. G.
Gospodinov, A.
Papamichos-Chronakis, M.
Affiliation
Cancer Research UK Newcastle Drug Discovery Unit, Newcastle University Centre for Cancer, Newcastle University, Paul O'Gorman Building, Newcastle upon Tyne, NE2 4HH, UK.Issue Date
2020
Metadata
Show full item recordAbstract
Collisions between the DNA replication machinery and co-transcriptional R-loops can impede DNA synthesis and are a major source of genomic instability in cancer cells. How cancer cells deal with R-loops to proliferate is poorly understood. Here we show that the ATP-dependent chromatin remodelling INO80 complex promotes resolution of R-loops to prevent replication-associated DNA damage in cancer cells. Depletion of INO80 in prostate cancer PC3 cells leads to increased R-loops. Overexpression of the RNA:DNA endonuclease RNAse H1 rescues the DNA synthesis defects and suppresses DNA damage caused by INO80 depletion. R-loops co-localize with and promote recruitment of INO80 to chromatin. Artificial tethering of INO80 to a LacO locus enabled turnover of R-loops in cis. Finally, counteracting R-loops by INO80 promotes proliferation and averts DNA damage-induced death in cancer cells. Our work suggests that INO80-dependent resolution of R-loops promotes DNA replication in the presence of transcription, thus enabling unlimited proliferation in cancers.Citation
Prendergast L, McClurg UL, Hristova R, Berlinguer-Palmini R, Greener S, Veitch K, et al. Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability. Nat Commun. 2020;11(1):4534.Journal
Nature CommunicationsDOI
10.1038/s41467-020-18306-xPubMed ID
32913330Additional Links
https://dx.doi.org/10.1038/s41467-020-18306-xType
ArticleLanguage
enae974a485f413a2113503eed53cd6c53
10.1038/s41467-020-18306-x