• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Investigating the importance of B cells and antibodies during Trichuris muris infection using the IgMi mouse

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Sahputra2020_Article_Investiga ...
    Size:
    3.609Mb
    Format:
    PDF
    Description:
    From UNPAYWALL
    Download
    Authors
    Sahputra, R.
    Murphy, E. A.
    Forman, R.
    Mair, I.
    Fadlullah, Muhammad Z H
    Waisman, A.
    Muller, W.
    Else, K. J.
    Affiliation
    Division of Infection, Immunity and Respiratory Medicine, Lydia Becker Institute for Immunology, The University of Manchester, Manchester, UK.
    Issue Date
    2020
    
    Metadata
    Show full item record
    Abstract
    The IgMi mouse has normal B cell development; its B cells express an IgM B cell receptor but cannot class switch or secrete antibody. Thus, the IgMi mouse offers a model system by which to dissect out antibody-dependent and antibody-independent B cell function. Here, we provide the first detailed characterisation of the IgMi mouse post-Trichuris muris (T. muris) infection, describing expulsion phenotype, cytokine production, gut pathology and changes in T regulatory cells, T follicular helper cells and germinal centre B cells, in addition to RNA sequencing (RNA seq) analyses of wild-type littermates (WT) and mutant B cells prior to and post infection. IgMi mice were susceptible to a high-dose infection, with reduced Th2 cytokines and elevated B cell-derived IL-10 in mesenteric lymph nodes (MLN) compared to controls. A low-dose infection regime revealed IgMi mice to have significantly more apoptotic cells in the gut compared to WT mice, but no change in intestinal inflammation. IL-10 levels were again elevated. Collectively, this study showcases the potential of the IgMi mouse as a tool for understanding B cell biology and suggests that the B cell plays both antibody-dependent and antibody-independent roles post high- and low-dose T. muris infection. KEY MESSAGES: During a high-dose T. muris infection, B cells are important in maintaining the Th1/Th2 balance in the MLN through an antibody-independent mechanism. High levels of IL-10 in the MLN early post-infection, and the presence of IL-10-producing B cells, correlates with susceptibility to T. muris infection. B cells maintain gut homeostasis during chronic T. muris infection via an antibody-dependent mechanism. Keywords: B cells; IgMi mouse; Interleukin-10; Intestinal pathology; Th1/Th2; Trichuris muris.
    Citation
    Sahputra R, Murphy EA, Forman R, Mair I, Fadlullah MZH, Waisman A, et al. Investigating the importance of B cells and antibodies during Trichuris muris infection using the IgMi mouse. J Mol Med (Berl). 2020;98(9):1301-17.
    Journal
    Journal of Molecular Medicine
    URI
    http://hdl.handle.net/10541/623273
    DOI
    10.1007/s00109-020-01954-3
    PubMed ID
    32778925
    Additional Links
    https://dx.doi.org/10.1007/s00109-020-01954-3
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.1007/s00109-020-01954-3
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • The Essential Role Played by B Cells in Supporting Protective Immunity Against Trichuris muris Infection Is by Controlling the Th1/Th2 Balance in the Mesenteric Lymph Nodes and Depends on Host Genetic Background.
    • Authors: Sahputra R, Ruckerl D, Couper KN, Muller W, Else KJ
    • Issue date: 2019
    • Increased susceptibility to oral Trichuris muris infection in the specific absence of CXCR5(+) CD11c(+) cells.
    • Authors: Bradford BM, Donaldson DS, Forman R, Else KJ, Mabbott NA
    • Issue date: 2018 Aug
    • Characterisation of the protective immune response following subcutaneous vaccination of susceptible mice against Trichuris muris.
    • Authors: Dixon H, Little MC, Else KJ
    • Issue date: 2010 May
    • Distinct DC subsets regulate adaptive Th1 and 2 responses during Trichuris muris infection.
    • Authors: Demiri M, Müller-Luda K, Agace WW, Svensson-Frej M
    • Issue date: 2017 Oct
    • B cells and antibodies are required for resistance to the parasitic gastrointestinal nematode Trichuris muris.
    • Authors: Blackwell NM, Else KJ
    • Issue date: 2001 Jun
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.