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    The biomolecule corona of lipid nanoparticles contains circulating cell-free DNA

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    Authors
    Gardner, Lois
    Warrington, Jessica
    Rogan, Jane
    Rothwell, Dominic G
    Brady, Ged
    Dive, Caroline
    Kostarelos, K.
    Hadjidemetriou, Marilena
    Affiliation
    Nanomedicine Lab, Faculty of Biology, Medicine and Health, The University of Manchester, AV Hill Building, Manchester, UK.
    Issue Date
    2020
    
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    Abstract
    The spontaneous adsorption of biomolecules onto the surface of nanoparticles (NPs) in complex physiological biofluids has been widely investigated over the last decade. Characterisation of the protein composition of the 'biomolecule corona' has dominated research efforts, whereas other classes of biomolecules, such as nucleic acids, have received no interest. Scarce, speculative statements exist in the literature about the presence of nucleic acids in the biomolecule corona, with no previous studies attempting to describe the contribution of genomic content to the blood-derived NP corona. Herein, we provide the first experimental evidence of the interaction of circulating cell-free DNA (cfDNA) with lipid-based NPs upon their incubation with human plasma samples, obtained from healthy volunteers and ovarian carcinoma patients. Our results also demonstrate an increased amount of detectable cfDNA in patients with cancer. Proteomic analysis of the same biomolecule coronas revealed the presence of histone proteins, suggesting an indirect, nucleosome-mediated NP-cfDNA interaction. The finding of cfDNA as part of the NP corona, offers a previously unreported new scope regarding the chemical composition of the 'biomolecule corona' and opens up new possibilities for the potential exploitation of the biomolecule corona for the enrichment and analysis of blood-circulating nucleic acids.
    Citation
    Gardner L, Warrington J, Rogan J, Rothwell DG, Brady G, Dive C, et al. The biomolecule corona of lipid nanoparticles contains circulating cell-free DNA. Nanoscale Horizons. 2020.
    Journal
    Nanoscale Horizons
    URI
    http://hdl.handle.net/10541/623244
    DOI
    10.1039/d0nh00333f
    PubMed ID
    32853302
    Additional Links
    https://dx.doi.org/10.1039/d0nh00333f
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.1039/d0nh00333f
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