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    Spatial proximity between T and PD-L1 expressing cells as a prognostic biomarker for oropharyngeal squamous cell carcinoma

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    Authors
    Tsakiroglou, AM
    Fergie, M
    Oguejiofor, K
    Linton, Kim M
    Thomson, David J
    Stern, Peter L
    Astley, S
    Byers, R
    West, Catharine ML
    Affiliation
    Division of Cancer Sciences, University of Manchester, Manchester, M13 9PG
    Issue Date
    2019
    
    Metadata
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    Abstract
    BACKGROUND: Fulfilling the promise of cancer immunotherapy requires novel predictive biomarkers to characterise the host immune microenvironment. Deciphering the complexity of immune cell interactions requires an automated multiplex approach to histological analysis of tumour sections. We tested a new automatic approach to select tissue and quantify the frequencies of cell-cell spatial interactions occurring in the PD1/PD-L1 pathway, hypothesised to reflect immune escape in oropharyngeal squamous cell carcinoma (OPSCC). METHODS: Single sections of diagnostic biopsies from 72 OPSCC patients were stained using multiplex immunofluorescence (CD8, PD1, PD-L1, CD68). Following multispectral scanning and automated regions-of-interest selection, the Hypothesised Interaction Distribution (HID) method quantified spatial proximity between cells. Method applicability was tested by investigating the prognostic significance of co-localised cells (within 30??m) in patients stratified by HPV status. RESULTS: High frequencies of proximal CD8+ and PD-L1+ (HR 2.95, p?=?0.025) and PD1+ and PD-L1+ (HR 2.64, p?=?0.042) cells were prognostic for poor overall survival in patients with HPV negative OPSCC (n?=?31). CONCLUSION: The HID method can quantify spatial interactions considered to reflect immune escape and generate prognostic information in OPSCC. The new automated approach is ready to test in additional cohorts and its applicability should be explored in research and clinical studies.
    Citation
    Tsakiroglou AM, Fergie M, Oguejiofor K, Linton K, Thomson D, Stern PL, et al. Spatial proximity between T and PD-L1 expressing cells as a prognostic biomarker for oropharyngeal squamous cell carcinoma. Br J Cancer. 2019:10.
    Journal
    British Journal of Cancer
    URI
    http://hdl.handle.net/10541/622630
    DOI
    10.1038/s41416-019-0634-z
    PubMed ID
    31806878
    Additional Links
    https://dx.doi.org/10.1038/s41416-019-0634-z
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41416-019-0634-z
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