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dc.contributor.authorMaya-Miles, D
dc.contributor.authorAndujar, E
dc.contributor.authorPerez-Alegre, M
dc.contributor.authorMurillo-Pineda, M
dc.contributor.authorBarrientos-Moreno, M
dc.contributor.authorCabello-Lobato, Maria J
dc.contributor.authorGomez-Marin, E
dc.contributor.authorMorillo-Huesca, M
dc.contributor.authorPrado, F
dc.date.accessioned2019-09-11T09:09:14Z
dc.date.available2019-09-11T09:09:14Z
dc.date.issued2019en
dc.identifier.citationMaya-Miles D, Andujar E, Perez-Alegre M, Murillo-Pineda M, Barrientos-Moreno M, Cabello-Lobato MJ, et al. Crosstalk between chromatin structure, cohesin activity and transcription. Epigenetics Chromatin. 2019 Jul 22;12(1):47.en
dc.identifier.pmid31331360en
dc.identifier.doi10.1186/s13072-019-0293-6en
dc.identifier.urihttp://hdl.handle.net/10541/621997
dc.description.abstractBACKGROUND: A complex interplay between chromatin and topological machineries is critical for genome architecture and function. However, little is known about these reciprocal interactions, even for cohesin, despite its multiple roles in DNA metabolism. RESULTS: We have used genome-wide analyses to address how cohesins and chromatin structure impact each other in yeast. Cohesin inactivation in scc1-73 mutants during the S and G2 phases causes specific changes in chromatin structure that preferentially take place at promoters; these changes include a significant increase in the occupancy of the - 1 and + 1 nucleosomes. In addition, cohesins play a major role in transcription regulation that is associated with specific promoter chromatin architecture. In scc1-73 cells, downregulated genes are enriched in promoters with short or no nucleosome-free region (NFR) and a fragile nucleosome - 1/RSC complex" particle. These results, together with a preferential increase in the occupancy of nucleosome - 1 of these genes, suggest that cohesins promote transcription activation by helping RSC to form the NFR. In sharp contrast, the scc1-73 upregulated genes are enriched in promoters with an "open" chromatin structure and are mostly at cohesin-enriched regions, suggesting that a local accumulation of cohesins might help to inhibit transcription. On the other hand, a dramatic loss of chromatin integrity by histone depletion during DNA replication has a moderate effect on the accumulation and distribution of cohesin peaks along the genome. CONCLUSIONS: Our analyses of the interplay between chromatin integrity and cohesin activity suggest that cohesins play a major role in transcription regulation, which is associated with specific chromatin architecture and cohesin-mediated nucleosome alterations of the regulated promoters. In contrast, chromatin integrity plays only a minor role in the binding and distribution of cohesins."en
dc.language.isoenen
dc.relation.urlhttps://dx.doi.org/10.1186/s13072-019-0293-6en
dc.titleCrosstalk between chromatin structure, cohesin activity and transcriptionen
dc.typeArticleen
dc.contributor.departmentDepartment of Genome Biology, Andalusian Molecular Biology and Regenerative Medicine (CABIMER), CSIC-University of Seville-University Pablo de Olavide, Seville, Spainen
dc.identifier.journalEpigenetics & Chromatinen
dc.description.noteen]
refterms.dateFOA2019-09-21T20:07:26Z


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