Mechanistic modelling supports entwined rather than exclusively competitive DNA double-strand break repair pathway
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Authors
Ingram, SWarmenhoven, J
Henthorn, Nicholas
Smith, E
Chadwick, A
Burnet, Neil G
Mackay, Ranald I
Kirkby, Norman
Kirkby, Karen J
Merchant, Michael J
Affiliation
Division of Cancer Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK.Issue Date
2019
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Following radiation induced DNA damage, several repair pathways are activated to help preserve genome integrity. Double Strand Breaks (DSBs), which are highly toxic, have specified repair pathways to address them. The main repair pathways used to resolve DSBs are Non-Homologous End Joining (NHEJ) and Homologous Recombination (HR). Cell cycle phase determines the availability of HR, but the repair choice between pathways in the G2 phases where both HR and NHEJ can operate is not clearly understood. This study compares several in silico models of repair choice to experimental data published in the literature, each model representing a different possible scenario describing how repair choice takes place. Competitive only scenarios, where initial protein recruitment determines repair choice, are unable to fit the literature data. In contrast, the scenario which uses a more entwined relationship between NHEJ and HR, incorporating protein co-localisation and RNF138-dependent removal of the Ku/DNA-PK complex, is better able to predict levels of repair similar to the experimental data. Furthermore, this study concludes that co-localisation of the Mre11-Rad50-Nbs1 (MRN) complexes, with initial NHEJ proteins must be modeled to accurately depict repair choice.Citation
Ingram SP, Warmenhoven JW, Henthorn NT, Smith EAK, Chadwick AL, Burnet NG, et al. Mechanistic modelling supports entwined rather than exclusively competitive DNA double-strand break repair pathway. Sci Rep. 2019; 9(1):6359.Journal
Scientific ReportsDOI
10.1038/s41598-019-42901-8PubMed ID
31015540Additional Links
https://dx.doi.org/10.1038/s41598-019-42901-8Type
ArticleLanguage
enae974a485f413a2113503eed53cd6c53
10.1038/s41598-019-42901-8