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dc.contributor.authorChen, S
dc.contributor.authorHuang, V
dc.contributor.authorXu, X
dc.contributor.authorLivingstone, J
dc.contributor.authorSoares, F
dc.contributor.authorJeon, J
dc.contributor.authorZeng, Y
dc.contributor.authorHua, JT
dc.contributor.authorPetricca, J
dc.contributor.authorGuo, H
dc.contributor.authorWang, M
dc.contributor.authorYousif, F
dc.contributor.authorZhang, Y
dc.contributor.authorDonmez, N
dc.contributor.authorAhmed, M
dc.contributor.authorVolik, S
dc.contributor.authorLapuk, A
dc.contributor.authorChua, MLK
dc.contributor.authorHeisler, LE
dc.contributor.authorFoucal, A
dc.contributor.authorFox, NS
dc.contributor.authorFraser, M
dc.contributor.authorBhandari, V
dc.contributor.authorShiah, YJ
dc.contributor.authorGuan, J
dc.contributor.authorLi, J
dc.contributor.authorOrain, M
dc.contributor.authorPicard, V
dc.contributor.authorHovington, H
dc.contributor.authorBergeron, A
dc.contributor.authorLacombe, L
dc.contributor.authorFradet, Y
dc.contributor.authorTetu, B
dc.contributor.authorLiu, S
dc.contributor.authorFeng, F
dc.contributor.authorWu, X
dc.contributor.authorShao, YW
dc.contributor.authorKomor, MA
dc.contributor.authorSahinalp, C
dc.contributor.authorCollins, C
dc.contributor.authorHoogstrate, Y
dc.contributor.authorde Jong, M
dc.contributor.authorFijneman, RJA
dc.contributor.authorFei, T
dc.contributor.authorJenster, G
dc.contributor.authorvan der Kwast, T
dc.contributor.authorBristow, Robert G
dc.contributor.authorBoutros, PC
dc.contributor.authorHe, HH
dc.date.accessioned2019-03-29T14:22:26Z
dc.date.available2019-03-29T14:22:26Z
dc.date.issued2019en
dc.identifier.citationChen S, Huang V, Xu X, Livingstone J, Soares F, Jeon J, et al. Widespread and functional RNA circularization in localized prostate cancer. Cell. 2019 Feb 7;176(4):831-43 e22.en
dc.identifier.pmid30735634en
dc.identifier.doi10.1016/j.cell.2019.01.025en
dc.identifier.urihttp://hdl.handle.net/10541/621643
dc.description.abstractThe cancer transcriptome is remarkably complex, including low-abundance transcripts, many not polyadenylated. To fully characterize the transcriptome of localized prostate cancer, we performed ultra-deep total RNA-seq on 144 tumors with rich clinical annotation. This revealed a linear transcriptomic subtype associated with the aggressive intraductal carcinoma sub-histology and a fusion profile that differentiates localized from metastatic disease. Analysis of back-splicing events showed widespread RNA circularization, with the average tumor expressing 7,232 circular RNAs (circRNAs). The degree of circRNA production was correlated to disease progression in multiple patient cohorts. Loss-of-function screening identified 11.3% of highly abundant circRNAs as essential for cell proliferation; for ?90% of these, their parental linear transcripts were not essential. Individual circRNAs can have distinct functions, with circCSNK1G3 promoting cell growth by interacting with miR-181. These data advocate for adoption of ultra-deep RNA-seq without poly-A selection to interrogate both linear and circular transcriptomes.en
dc.language.isoenen
dc.relation.urlhttps://dx.doi.org/10.1016/j.cell.2019.01.025en
dc.titleWidespread and functional RNA circularization in localized prostate canceren
dc.typeArticleen
dc.contributor.departmentDepartment of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada;en
dc.identifier.journalCellen
dc.description.noteen]


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