• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Effect of pembrolizumab on CD4(+) CD25(+) , CD4(+) LAP(+) and CD4(+) TIM-3(+) T cell subsets.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Toor, SM
    Sasidharan, N
    Pfister, G
    Elkord, Eyad
    Affiliation
    Cancer Research Center, Qatar, Biomedical Research Institute, Hamad Bin Khalifa University, Qatar Foundation, Doha, Qatar
    Issue Date
    2019
    
    Metadata
    Show full item record
    Abstract
    Tumor immune evasion involves the expansion of avidly proliferating immunosuppressive cells and inhibition of effector T cell proliferation. Immune checkpoints (IC) block the activation pathways of tumor-reactive T cells. IC pathways are often exploited by tumor cells to evade immune destruction, and blocking these pathways through IC inhibitors (ICI) has shown promising results in multiple malignancies. In this study, we investigated the effects of an ICI, pembrolizumab, on various T cell subsets in vitro. We compared the suppressive activity of CD4+ CD25+ regulatory T cells (conventional Treg ) with T cells expressing T cell immunoglobulin-3+ (TIM-3+ ) and latency-associated peptide (LAP)+ T cells. We found that LAP-expressing T cells were more suppressive than conventional Treg , but TIM-3-expressing T cells were not suppressive. Our results show that pembrolizumab does not modulate functions of Treg and mediates its immunostimulatory effects via the release of effector T cells from suppression. These findings may assist in the development of agents designed to intervene in IC pathways to overcome Treg resistance to ICI.
    Citation
    Toor SM, Sasidharan Nair V, Pfister G, Elkord E. Effect of pembrolizumab on CD4(+) CD25(+) , CD4(+) LAP(+) and CD4(+) TIM-3(+) T cell subsets. Clin Exp Immunol. 2019 Jan 28.
    Journal
    Clinical and Experimental Immunology
    URI
    http://hdl.handle.net/10541/621611
    DOI
    10.1111/cei.13264
    PubMed ID
    30693485
    Additional Links
    https://dx.doi.org/10.1111/cei.13264
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.1111/cei.13264
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • In-vitro effect of pembrolizumab on different T regulatory cell subsets.
    • Authors: Toor SM, Syed Khaja AS, Alkurd I, Elkord E
    • Issue date: 2018 Feb
    • Increased frequency and suppressive activity of CD127(low/-) regulatory T cells in the peripheral circulation of patients with head and neck squamous cell carcinoma are associated with advanced stage and nodal involvement.
    • Authors: Drennan S, Stafford ND, Greenman J, Green VL
    • Issue date: 2013 Nov
    • Blockade of TGF-β signaling to enhance the antitumor response is accompanied by dysregulation of the functional activity of CD4(+)CD25(+)Foxp3(+) and CD4(+)CD25(-)Foxp3(+) T cells.
    • Authors: Polanczyk MJ, Walker E, Haley D, Guerrouahen BS, Akporiaye ET
    • Issue date: 2019 Jul 9
    • A specific CD4 epitope bound by tregalizumab mediates activation of regulatory T cells by a unique signaling pathway.
    • Authors: Helling B, König M, Dälken B, Engling A, Krömer W, Heim K, Wallmeier H, Haas J, Wildemann B, Fritz B, Jonuleit H, Kubach J, Dingermann T, Radeke HH, Osterroth F, Uherek C, Czeloth N, Schüttrumpf J
    • Issue date: 2015 Apr
    • CD69+ CD4+ CD25- T cells, a new subset of regulatory T cells, suppress T cell proliferation through membrane-bound TGF-beta 1.
    • Authors: Han Y, Guo Q, Zhang M, Chen Z, Cao X
    • Issue date: 2009 Jan 1
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.