The human in vivo biomolecule corona onto PEGylated Liposomes: a proof-of-concept clinical study.
Smith, Duncan L
Clamp, Andrew R
AffiliationNanomedicine Lab, Faculty of Biology, Medicine and Health, The University of Manchester, AV Hill Building, Manchester, M13 9PT, UK
MetadataShow full item record
AbstractThe self-assembled layered adsorption of proteins onto nanoparticle (NP) surfaces, once in contact with biological fluids, is termed the "protein corona" and it is gradually seen as a determinant factor for the overall biological behavior of NPs. Here, the previously unreported in vivo protein corona formed in human systemic circulation is described. The human-derived protein corona formed onto PEGylated doxorubicin-encapsulated liposomes (Caelyx) is thoroughly characterized following the recovery of liposomes from the blood circulation of ovarian carcinoma patients. In agreement with previous investigations in mice, the in vivo corona is found to be molecularly richer in comparison to its counterpart ex vivo corona. The intravenously infused liposomes are able to scavenge the blood pool and surface-capture low-molecular-weight, low-abundance plasma proteins that cannot be detected by conventional plasma proteomic analysis. This study describes the previously elusive or postulated formation of protein corona around nanoparticles in vivo in humans and illustrates that it can potentially be used as a novel tool to analyze the blood circulation proteome.
CitationHadjidemetriou M, McAdam S, Garner G, Thackeray C, Knight D, Smith D, et al. The human in vivo biomolecule corona onto pegylated liposomes: a proof-of-concept clinical study. Adv Mater. 2018 Nov 28:e1803335.
- In Vivo Biomolecule Corona around Blood-Circulating, Clinically Used and Antibody-Targeted Lipid Bilayer Nanoscale Vesicles.
- Authors: Hadjidemetriou M, Al-Ahmady Z, Mazza M, Collins RF, Dawson K, Kostarelos K
- Issue date: 2015 Aug 25
- Time-evolution of in vivo protein corona onto blood-circulating PEGylated liposomal doxorubicin (DOXIL) nanoparticles.
- Authors: Hadjidemetriou M, Al-Ahmady Z, Kostarelos K
- Issue date: 2016 Apr 7
- Impact of the biomolecular corona on the structure of PEGylated liposomes.
- Authors: Digiacomo L, Pozzi D, Amenitsch H, Caracciolo G
- Issue date: 2017 Aug 22
- A novel scavenging tool for cancer biomarker discovery based on the blood-circulating nanoparticle protein corona.
- Authors: Hadjidemetriou M, Al-Ahmady Z, Buggio M, Swift J, Kostarelos K
- Issue date: 2019 Jan
- Multi-parametric surface plasmon resonance platform for studying liposome-serum interactions and protein corona formation.
- Authors: Kari OK, Rojalin T, Salmaso S, Barattin M, Jarva H, Meri S, Yliperttula M, Viitala T, Urtti A
- Issue date: 2017 Apr