Early human hemogenic endothelium generates primitive and definitive hematopoiesis in vitro.
Authors
Garcia-Alegria, EMenegatti, S
Fadlullah, Muhammad Z H
Menendez, P
Lacaud, Georges
Kouskoff, Valerie
Affiliation
Developmental Haematopoiesis Group, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester M13 9PT, UKIssue Date
2018
Metadata
Show full item recordAbstract
The differentiation of human embryonic stem cells (hESCs) to hematopoietic lineages initiates with the specification of hemogenic endothelium, a transient specialized endothelial precursor of all blood cells. This in vitro system provides an invaluable model to dissect the emergence of hematopoiesis in humans. However, the study of hematopoiesis specification is hampered by a lack of consensus in the timing of hemogenic endothelium analysis and the full hematopoietic potential of this population. Here, our data reveal a sharp decline in the hemogenic potential of endothelium populations isolated over the course of hESC differentiation. Furthermore, by tracking the dynamic expression of CD31 and CD235a at the onset of hematopoiesis, we identified three populations of hematopoietic progenitors, representing primitive and definitive subsets that all emerge from the earliest specified hemogenic endothelium. Our data establish that hemogenic endothelium populations endowed with primitive and definitive hematopoietic potential are specified simultaneously from the mesoderm in differentiating hESCs.Citation
Garcia-Alegria E, Menegatti S, Fadlullah MZH, Menendez P, Lacaud G, Kouskoff V. Early human hemogenic endothelium generates primitive and definitive hematopoiesis in vitro. Stem Cell Reports. 2018 Nov 13;11(5):1061-74.Journal
Stem Cell ReportsDOI
10.1016/j.stemcr.2018.09.013PubMed ID
30449319Additional Links
https://dx.doi.org/10.1016/j.stemcr.2018.09.013Type
ArticleLanguage
enae974a485f413a2113503eed53cd6c53
10.1016/j.stemcr.2018.09.013
Scopus Count
Collections
Related articles
- CD1d expression demarcates CDX4+ hemogenic mesoderm with definitive hematopoietic potential.
- Authors: Philip Creamer J, Luff SA, Yu H, Sturgeon CM
- Issue date: 2022 Jul
- Directed differentiation of definitive hemogenic endothelium and hematopoietic progenitors from human pluripotent stem cells.
- Authors: Ditadi A, Sturgeon CM
- Issue date: 2016 May 15
- Cytokine-free directed differentiation of human pluripotent stem cells efficiently produces hemogenic endothelium with lymphoid potential.
- Authors: Galat Y, Dambaeva S, Elcheva I, Khanolkar A, Beaman K, Iannaccone PM, Galat V
- Issue date: 2017 Mar 17
- ETV2 expression marks blood and endothelium precursors, including hemogenic endothelium, at the onset of blood development.
- Authors: Wareing S, Eliades A, Lacaud G, Kouskoff V
- Issue date: 2012 Sep
- In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation.
- Authors: Garcia-Alegria E, Potts B, Menegatti S, Kouskoff V
- Issue date: 2021 Mar 19