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    Early human hemogenic endothelium generates primitive and definitive hematopoiesis in vitro.

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    Authors
    Garcia-Alegria, E
    Menegatti, S
    Fadlullah, Muhammad Z H
    Menendez, P
    Lacaud, Georges
    Kouskoff, Valerie
    Affiliation
    Developmental Haematopoiesis Group, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester M13 9PT, UK
    Issue Date
    2018
    
    Metadata
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    Abstract
    The differentiation of human embryonic stem cells (hESCs) to hematopoietic lineages initiates with the specification of hemogenic endothelium, a transient specialized endothelial precursor of all blood cells. This in vitro system provides an invaluable model to dissect the emergence of hematopoiesis in humans. However, the study of hematopoiesis specification is hampered by a lack of consensus in the timing of hemogenic endothelium analysis and the full hematopoietic potential of this population. Here, our data reveal a sharp decline in the hemogenic potential of endothelium populations isolated over the course of hESC differentiation. Furthermore, by tracking the dynamic expression of CD31 and CD235a at the onset of hematopoiesis, we identified three populations of hematopoietic progenitors, representing primitive and definitive subsets that all emerge from the earliest specified hemogenic endothelium. Our data establish that hemogenic endothelium populations endowed with primitive and definitive hematopoietic potential are specified simultaneously from the mesoderm in differentiating hESCs.
    Citation
    Garcia-Alegria E, Menegatti S, Fadlullah MZH, Menendez P, Lacaud G, Kouskoff V. Early human hemogenic endothelium generates primitive and definitive hematopoiesis in vitro. Stem Cell Reports. 2018 Nov 13;11(5):1061-74.
    Journal
    Stem Cell Reports
    URI
    http://hdl.handle.net/10541/621382
    DOI
    10.1016/j.stemcr.2018.09.013
    PubMed ID
    30449319
    Additional Links
    https://dx.doi.org/10.1016/j.stemcr.2018.09.013
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.stemcr.2018.09.013
    Scopus Count
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    All Paterson Institute for Cancer Research

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