Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta.
van Oudenaarden, A
AffiliationHubrecht Institute-KNAW, University Medical Center Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
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AbstractHaematopoietic stem cells (HSCs) are generated from haemogenic endothelial (HE) cells via the formation of intra-aortic haematopoietic clusters (IAHCs) in vertebrate embryos. The molecular events controlling endothelial specification, endothelial-to-haematopoietic transition (EHT) and IAHC formation, as it occurs in vivo inside the aorta, are still poorly understood. To gain insight in these processes, we performed single-cell RNA-sequencing of non-HE cells, HE cells, cells undergoing EHT, IAHC cells, and whole IAHCs isolated from mouse embryo aortas. Our analysis identified the genes and transcription factor networks activated during the endothelial-to-haematopoietic switch and IAHC cell maturation toward an HSC fate. Our study provides an unprecedented complete resource to study in depth HSC generation in vivo. It will pave the way for improving HSC production in vitro to address the growing need for tailor-made HSCs to treat patients with blood-related disorders.
CitationSingle-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta. 2018, 9(1): 2517 Nat Commun
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- Authors: Thambyrajah R, Mazan M, Patel R, Moignard V, Stefanska M, Marinopoulou E, Li Y, Lancrin C, Clapes T, Möröy T, Robin C, Miller C, Cowley S, Göttgens B, Kouskoff V, Lacaud G
- Issue date: 2016 Jan
- The embryonic origins and genetic programming of emerging haematopoietic stem cells.
- Authors: Ciau-Uitz A, Patient R
- Issue date: 2016 Nov
- Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level.
- Authors: Swiers G, Baumann C, O'Rourke J, Giannoulatou E, Taylor S, Joshi A, Moignard V, Pina C, Bee T, Kokkaliaris KD, Yoshimoto M, Yoder MC, Frampton J, Schroeder T, Enver T, Göttgens B, de Bruijn MFTR
- Issue date: 2013
- In vivo imaging of haematopoietic cells emerging from the mouse aortic endothelium.
- Authors: Boisset JC, van Cappellen W, Andrieu-Soler C, Galjart N, Dzierzak E, Robin C
- Issue date: 2010 Mar 4