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    Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta.

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    Authors
    Baron, C
    Kester, L
    Klaus, A
    Boisset, J
    Thambyrajah, Roshana
    Yvernogeau, L
    Kouskoff, V
    Lacaud, Georges
    van Oudenaarden, A
    Robin, C
    Affiliation
    Hubrecht Institute-KNAW, University Medical Center Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands
    Issue Date
    2018-06-28
    
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    Abstract
    Haematopoietic stem cells (HSCs) are generated from haemogenic endothelial (HE) cells via the formation of intra-aortic haematopoietic clusters (IAHCs) in vertebrate embryos. The molecular events controlling endothelial specification, endothelial-to-haematopoietic transition (EHT) and IAHC formation, as it occurs in vivo inside the aorta, are still poorly understood. To gain insight in these processes, we performed single-cell RNA-sequencing of non-HE cells, HE cells, cells undergoing EHT, IAHC cells, and whole IAHCs isolated from mouse embryo aortas. Our analysis identified the genes and transcription factor networks activated during the endothelial-to-haematopoietic switch and IAHC cell maturation toward an HSC fate. Our study provides an unprecedented complete resource to study in depth HSC generation in vivo. It will pave the way for improving HSC production in vitro to address the growing need for tailor-made HSCs to treat patients with blood-related disorders.
    Citation
    Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta. 2018, 9(1): 2517 Nat Commun
    Journal
    Nature Communications
    URI
    http://hdl.handle.net/10541/621161
    DOI
    10.1038/s41467-018-04893-3
    PubMed ID
    29955049
    Type
    Article
    Language
    en
    ISSN
    2041-1723
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41467-018-04893-3
    Scopus Count
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    All Paterson Institute for Cancer Research

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