Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta.
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Authors
Baron, CKester, L
Klaus, A
Boisset, J
Thambyrajah, Roshana
Yvernogeau, L
Kouskoff, V
Lacaud, Georges
van Oudenaarden, A
Robin, C
Affiliation
Hubrecht Institute-KNAW, University Medical Center Utrecht, Uppsalalaan 8, 3584 CT, Utrecht, The NetherlandsIssue Date
2018-06-28
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Haematopoietic stem cells (HSCs) are generated from haemogenic endothelial (HE) cells via the formation of intra-aortic haematopoietic clusters (IAHCs) in vertebrate embryos. The molecular events controlling endothelial specification, endothelial-to-haematopoietic transition (EHT) and IAHC formation, as it occurs in vivo inside the aorta, are still poorly understood. To gain insight in these processes, we performed single-cell RNA-sequencing of non-HE cells, HE cells, cells undergoing EHT, IAHC cells, and whole IAHCs isolated from mouse embryo aortas. Our analysis identified the genes and transcription factor networks activated during the endothelial-to-haematopoietic switch and IAHC cell maturation toward an HSC fate. Our study provides an unprecedented complete resource to study in depth HSC generation in vivo. It will pave the way for improving HSC production in vitro to address the growing need for tailor-made HSCs to treat patients with blood-related disorders.Citation
Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta. 2018, 9(1): 2517 Nat CommunJournal
Nature CommunicationsDOI
10.1038/s41467-018-04893-3PubMed ID
29955049Type
ArticleLanguage
enISSN
2041-1723ae974a485f413a2113503eed53cd6c53
10.1038/s41467-018-04893-3
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