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    Enhancer activation by pharmacologic displacement of LSD1 from GFI1 induces differentiation in acute myeloid leukemia.

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    Authors
    Maiques-Diaz, Alba
    Spencer, Gary J
    Lynch, James T
    Ciceri, Filippo
    Williams, Emma L
    Amaral, Fabio
    Wiseman, Daniel H
    Harris, William J
    Li, Yaoyong
    Sahoo, Sudhakar
    Hitchin, James R
    Mould, Daniel P
    Fairweather, Emma E
    Waszkowycz, Bohdan
    Jordan, Allan M
    Smith, Duncan L
    Somervaille, Tim CP
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    Affiliation
    Leukaemia Biology Laboratory, Cancer Research UK Manchester Institute, The University of Manchester, Manchester Cancer Research Centre Building, 555 Wilmslow Road, Manchester M20 4GJ,
    Issue Date
    2018-03-27
    
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    Abstract
    Pharmacologic inhibition of LSD1 promotes blast cell differentiation in acute myeloid leukemia (AML) with MLL translocations. The assumption has been that differentiation is induced through blockade of LSD1's histone demethylase activity. However, we observed that rapid, extensive, drug-induced changes in transcription occurred without genome-wide accumulation of the histone modifications targeted for demethylation by LSD1 at sites of LSD1 binding and that a demethylase-defective mutant rescued LSD1 knockdown AML cells as efficiently as wild-type protein. Rather, LSD1 inhibitors disrupt the interaction of LSD1 and RCOR1 with the SNAG-domain transcription repressor GFI1, which is bound to a discrete set of enhancers located close to transcription factor genes that regulate myeloid differentiation. Physical separation of LSD1/RCOR1 from GFI1 is required for drug-induced differentiation. The consequent inactivation of GFI1 leads to increased enhancer histone acetylation within hours, which directly correlates with the upregulation of nearby subordinate genes.
    Citation
    Enhancer activation by pharmacologic displacement of LSD1 from GFI1 induces differentiation in acute myeloid leukemia. 2018, 22(13): 3641-3659 Cell Rep
    Journal
    Cell Reports
    URI
    http://hdl.handle.net/10541/620985
    DOI
    10.1016/j.celrep.2018.03.012
    PubMed ID
    29590629
    Type
    Article
    Language
    en
    ISSN
    2211-1247
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.celrep.2018.03.012
    Scopus Count
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    All Paterson Institute for Cancer Research

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