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dc.contributor.authorLi, H
dc.contributor.authorSong, X
dc.contributor.authorYang, F
dc.contributor.authorBao, H
dc.contributor.authorLu, X
dc.contributor.authorPerez-Campo, Flor-Maria
dc.contributor.authorZhao, J
dc.date.accessioned2017-12-14T15:10:05Z
dc.date.available2017-12-14T15:10:05Z
dc.date.issued2018-01
dc.identifier.citationApplication of oligonucleotides to construct a conditional targeting vector for porcine IκBα. 2018, 17 (1):653-659 Mol Med Repen
dc.identifier.issn1791-3004
dc.identifier.pmid29115518
dc.identifier.doi10.3892/mmr.2017.7917
dc.identifier.urihttp://hdl.handle.net/10541/620716
dc.description.abstractConditional gene targeting at porcine IκBα may be a solution to delayed xenograft rejection, the main barrier to xenotransplantation. An oligonucleotide‑based method was applied to construct the vector for conditional targeting of porcine IκBα. This method was free from PCR amplification during the assembling of the different vector elements, avoiding introduction of unwanted mutations. With the help of short double‑stranded DNA fragments produced by annealing oligonucleotides, nondirectional cloning has also been avoided. By making the best of directional cloning, a highly complex targeting vector was built within 3 weeks. The present study also explained why the two recombination‑based methods (recombineering and gateway recombination), although having demonstrated to be highly efficient in constructing ordinary targeting vectors, were not appropriate in this context. The description in the present study of an additional method to efficiently construct targeting vectors is suggested to introduce more flexibility in the field therefore helping to meet the different needs of the researchers.
dc.language.isoenen
dc.rightsArchived with thanks to Molecular medicine reportsen
dc.titleApplication of oligonucleotides to construct a conditional targeting vector for porcine IκBα.en
dc.typeArticleen
dc.contributor.departmentCollege of Animal Science, Qingdao Agricultural University, Qingdao, Shandong 266109, P.R. Chinaen
dc.identifier.journalMolecular Medicine Reportsen
html.description.abstractConditional gene targeting at porcine IκBα may be a solution to delayed xenograft rejection, the main barrier to xenotransplantation. An oligonucleotide‑based method was applied to construct the vector for conditional targeting of porcine IκBα. This method was free from PCR amplification during the assembling of the different vector elements, avoiding introduction of unwanted mutations. With the help of short double‑stranded DNA fragments produced by annealing oligonucleotides, nondirectional cloning has also been avoided. By making the best of directional cloning, a highly complex targeting vector was built within 3 weeks. The present study also explained why the two recombination‑based methods (recombineering and gateway recombination), although having demonstrated to be highly efficient in constructing ordinary targeting vectors, were not appropriate in this context. The description in the present study of an additional method to efficiently construct targeting vectors is suggested to introduce more flexibility in the field therefore helping to meet the different needs of the researchers.


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