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    Primitive erythrocytes are generated from hemogenic endothelial cells

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    Authors
    Stefanska, Monika
    Batta, Kiran
    Patel, Rahima
    Florkowska, Magdalena
    Kouskoff, Valerie
    Lacaud, Georges
    Affiliation
    Cancer Research UK Stem Cell Biology Group, Cancer Research UK
    Issue Date
    2017-07-25
    
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    Abstract
    Primitive erythroblasts are the first blood cells generated during embryonic hematopoiesis. Tracking their emergence both in vivo and in vitro has remained challenging due to the lack of specific cell surface markers. To selectively investigate primitive erythropoiesis, we have engineered a new transgenic embryonic stem (ES) cell line, where eGFP expression is driven by the regulatory sequences of the embryonic βH1 hemoglobin gene expressed specifically in primitive erythroid cells. Using this ES cell line, we observed that the first primitive erythroblasts are detected in vitro around day 1.5 of blast colony differentiation, within the cell population positive for the early hematopoietic progenitor marker CD41. Moreover, we establish that these eGFP(+) cells emerge from a hemogenic endothelial cell population similarly to their definitive hematopoietic counterparts. We further generated a corresponding βH1-eGFP transgenic mouse model and demonstrated the presence of a primitive erythroid primed hemogenic endothelial cell population in the developing embryo. Taken together, our findings demonstrate that both in vivo and in vitro primitive erythrocytes are generated from hemogenic endothelial cells.
    Citation
    Primitive erythrocytes are generated from hemogenic endothelial cells. 2017, 7 (1):6401 Sci Rep
    Journal
    Scientific Reports
    URI
    http://hdl.handle.net/10541/620553
    DOI
    10.1038/s41598-017-06627-9
    PubMed ID
    28743905
    Type
    Meetings and Proceedings
    Language
    en
    ISSN
    2045-2322
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41598-017-06627-9
    Scopus Count
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    All Paterson Institute for Cancer Research

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