Primitive erythrocytes are generated from hemogenic endothelial cells
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Stefanska, MonikaBatta, Kiran
Patel, Rahima
Florkowska, Magdalena
Kouskoff, Valerie
Lacaud, Georges
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Cancer Research UK Stem Cell Biology Group, Cancer Research UKIssue Date
2017-07-25
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Primitive erythroblasts are the first blood cells generated during embryonic hematopoiesis. Tracking their emergence both in vivo and in vitro has remained challenging due to the lack of specific cell surface markers. To selectively investigate primitive erythropoiesis, we have engineered a new transgenic embryonic stem (ES) cell line, where eGFP expression is driven by the regulatory sequences of the embryonic βH1 hemoglobin gene expressed specifically in primitive erythroid cells. Using this ES cell line, we observed that the first primitive erythroblasts are detected in vitro around day 1.5 of blast colony differentiation, within the cell population positive for the early hematopoietic progenitor marker CD41. Moreover, we establish that these eGFP(+) cells emerge from a hemogenic endothelial cell population similarly to their definitive hematopoietic counterparts. We further generated a corresponding βH1-eGFP transgenic mouse model and demonstrated the presence of a primitive erythroid primed hemogenic endothelial cell population in the developing embryo. Taken together, our findings demonstrate that both in vivo and in vitro primitive erythrocytes are generated from hemogenic endothelial cells.Citation
Primitive erythrocytes are generated from hemogenic endothelial cells. 2017, 7 (1):6401 Sci RepJournal
Scientific ReportsDOI
10.1038/s41598-017-06627-9PubMed ID
28743905Type
Meetings and ProceedingsLanguage
enISSN
2045-2322ae974a485f413a2113503eed53cd6c53
10.1038/s41598-017-06627-9
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