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    Reference miRNAs for colorectal cancer: analysis and verification of current data.

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    Authors
    Danese, E
    Minicozzi, Annamaria
    Benati, M
    Paviati, E
    Lima-Oliveira, G
    Gusella, M
    Pasini, F
    Salvagno, G
    Montagnana, M
    Lippi, G
    Affiliation
    Clinical Biochemistry section, Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, Verona, Italy.
    Issue Date
    2017-08-21
    
    Metadata
    Show full item record
    Abstract
    MicroRNAs (miRNAs) hold great promise in cancer research. The use of appropriate reference miRNAs for normalization of qPCR data is crucial for accurate expression analysis. We present here analysis and verification of current data, proposing a workflow strategy for identification of reference miRNAs in colorectal cancer (CRC). We performed a systematic review of studies aimed to identify stable reference miRNAs in CRC through high-throughput screening. Among the candidate miRNAs selected from the literature we excluded those predicted to target oncogenes or tumor suppressor gene. We then assessed the expression levels of the remaining candidates in exosomes, plasma and tissue samples from CRC patients and healthy controls. The expression stability was evaluated by box-plot, ∆Cq analysis, NormFinder and BestKeeper statistical algorithms. The effects of normalisers on the relative quantification of the oncogenic miR-1290 was also assessed. Our results consistently showed that different combinations of miR-520d, miR-1228 and miR-345 provided the most stably expressed reference miRNAs in the three biological matrices. We identified suitable reference miRNAs for future miRNA expression studies in exosomes plasma and tissues CRC samples. We also provided a novel conceptual framework that overcome the need of performing ex novo identification of suitable reference genes in single experimental systems.
    Citation
    Reference miRNAs for colorectal cancer: analysis and verification of current data. 2017, 7 (1):8413 Sci Rep
    Journal
    Scientific Reports
    URI
    http://hdl.handle.net/10541/620535
    DOI
    10.1038/s41598-017-08784-3
    PubMed ID
    28827728
    Type
    Article
    Language
    en
    ISSN
    2045-2322
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41598-017-08784-3
    Scopus Count
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