Robotic mammosphere assay for high-throughput screening in triple-negative breast cancer.
Affiliation
Sahlgrenska Cancer Center, Goteborgs Universitet, Goteborg, SwedenIssue Date
2017-02-01
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In order to identify novel treatment principles specifically affecting cancer stem cells in triple-negative breast cancer, we have developed a high-throughput screening method based on the mammosphere and anoikis resistance assays allowing us to screen compounds using a functional readout. The assay was validated against manual protocols and through the use of positive controls, such as the response to hypoxia and treatment with the known cancer stem cell-targeting compound salinomycin. Manual and robotic procedures were compared and produced similar results in cell handling, cell cultures, and counting techniques, with no statistically significant difference produced from either method. The variance between samples processed manually versus robotically was no greater than 0.012, while Levene's test of significance was 0.2, indicating no significant difference between mammosphere data produced manually or robotically. Through the screening of 989 FDA-approved drugs and a follow-up screen assessing the antineoplastic subgroup, we have identified three therapeutic compounds with the ability to modulate the breast cancer stem cell fraction in the triple-negative breast cancer cell line MDA-MB-231, highlighting their potential usage as stem cell-specific adjuvant treatments.Citation
Robotic mammosphere assay for high-throughput screening in triple-negative breast cancer. 2017, SLAS DiscovJournal
SLAS DiscoveryDOI
10.1177/2472555217692321PubMed ID
28346100Type
ArticleLanguage
enISSN
2472-5560ae974a485f413a2113503eed53cd6c53
10.1177/2472555217692321
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