Preparation of protein extracts from Schizosaccharomyces pombe using trichloroacetic acid precipitation.
Affiliation
Agnes.Grallert@cruk.manchester.ac.uk Iain.Hagan@cruk.manchester.ac.ukCRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX, United KingdomIssue Date
2017-02-01
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Schizosaccharomyces pombe is an attractive model organism with which to study core principles of conserved molecular cell biology processes. The ability to monitor protein behavior following separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) underpins much of this activity. Here we describe a robust protocol for the preparation of protein samples for analysis by SDS-PAGE.Citation
Preparation of protein extracts from Schizosaccharomyces pombe using trichloroacetic acid precipitation. 2017, Cold Spring Harb ProtocJournal
Cold Spring Harbor ProtocolsDOI
10.1101/pdb.prot091579PubMed ID
28148851Type
ArticleLanguage
enISSN
1559-6095ae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot091579
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