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    Preparation of protein extracts from Schizosaccharomyces pombe using trichloroacetic acid precipitation.

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    Authors
    Grallert, Agnes
    Hagan, Iain M
    Affiliation
    Agnes.Grallert@cruk.manchester.ac.uk Iain.Hagan@cruk.manchester.ac.ukCRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX, United Kingdom
    Issue Date
    2017-02-01
    
    Metadata
    Show full item record
    Abstract
    Schizosaccharomyces pombe is an attractive model organism with which to study core principles of conserved molecular cell biology processes. The ability to monitor protein behavior following separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) underpins much of this activity. Here we describe a robust protocol for the preparation of protein samples for analysis by SDS-PAGE.
    Citation
    Preparation of protein extracts from Schizosaccharomyces pombe using trichloroacetic acid precipitation. 2017, Cold Spring Harb Protoc
    Journal
    Cold Spring Harbor Protocols
    URI
    http://hdl.handle.net/10541/620187
    DOI
    10.1101/pdb.prot091579
    PubMed ID
    28148851
    Type
    Article
    Language
    en
    ISSN
    1559-6095
    ae974a485f413a2113503eed53cd6c53
    10.1101/pdb.prot091579
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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