Robust, universal biomarker assay to detect senescent cells in biological specimens.
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Authors
Evangelou, KLougiakis, N
Rizou, S
Kotsinas, A
Kletsas, D
Muñoz-Espín, D
Kastrinakis, N
Pouli, N
Marakos, P
Townsend, Paul A
Serrano, M
Bartek, J
Gorgoulis, Vassilis G
Affiliation
Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, GreeceIssue Date
2017-02
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Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.Citation
Robust, universal biomarker assay to detect senescent cells in biological specimens. 2017, 16(1):192-197 Aging CellJournal
Aging CellDOI
10.1111/acel.12545PubMed ID
28165661Type
ArticleLanguage
enISSN
1474-9726ae974a485f413a2113503eed53cd6c53
10.1111/acel.12545
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