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dc.contributor.authorEvangelou, K
dc.contributor.authorGorgoulis, Vassilis G
dc.date.accessioned2016-12-09T22:01:05Z
dc.date.available2016-12-09T22:01:05Z
dc.date.issued2017
dc.identifier.citationSudan Black B, the specific histochemical stain for lipofuscin: a novel method to detect senescent cells. 2017, 1534:111-119 Methods Mol. Biol.en
dc.identifier.issn1940-6029
dc.identifier.pmid27812872
dc.identifier.doi10.1007/978-1-4939-6670-7_10
dc.identifier.urihttp://hdl.handle.net/10541/620043
dc.description.abstractThe Sudan-Black-B (SBB) histochemical stain is well known to specifically react against lipofuscin, an aggregate of oxidized proteins, lipids, and metals. Lipofuscin is related to many ageing processes. It is also known to accumulate in senescent cells. We recently proved that lipofuscin detection, when applying the SBB staining, is highly specific for the visualization of senescent cells. Here, we present in detail this SBB method that can detect senescent cells in any material, irrespective of its preparation. This provides unique advantages not only in understanding physiological processes and the pathophysiology of various diseases but also in estimating the response to therapeutic interventions.
dc.language.isoenen
dc.rightsArchived with thanks to Methods in molecular biology (Clifton, N.J.)en
dc.titleSudan Black B, the specific histochemical stain for lipofuscin: a novel method to detect senescent cells.en
dc.typeArticleen
dc.contributor.departmentMolecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Mikras Asias 75, Athens, 11527, Greeceen
dc.identifier.journalMethods in Molecular Biologyen
html.description.abstractThe Sudan-Black-B (SBB) histochemical stain is well known to specifically react against lipofuscin, an aggregate of oxidized proteins, lipids, and metals. Lipofuscin is related to many ageing processes. It is also known to accumulate in senescent cells. We recently proved that lipofuscin detection, when applying the SBB staining, is highly specific for the visualization of senescent cells. Here, we present in detail this SBB method that can detect senescent cells in any material, irrespective of its preparation. This provides unique advantages not only in understanding physiological processes and the pathophysiology of various diseases but also in estimating the response to therapeutic interventions.


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