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    Sudan Black B, the specific histochemical stain for lipofuscin: a novel method to detect senescent cells.

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    Authors
    Evangelou, K
    Gorgoulis, Vassilis G
    Affiliation
    Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Mikras Asias 75, Athens, 11527, Greece
    Issue Date
    2017
    
    Metadata
    Show full item record
    Abstract
    The Sudan-Black-B (SBB) histochemical stain is well known to specifically react against lipofuscin, an aggregate of oxidized proteins, lipids, and metals. Lipofuscin is related to many ageing processes. It is also known to accumulate in senescent cells. We recently proved that lipofuscin detection, when applying the SBB staining, is highly specific for the visualization of senescent cells. Here, we present in detail this SBB method that can detect senescent cells in any material, irrespective of its preparation. This provides unique advantages not only in understanding physiological processes and the pathophysiology of various diseases but also in estimating the response to therapeutic interventions.
    Citation
    Sudan Black B, the specific histochemical stain for lipofuscin: a novel method to detect senescent cells. 2017, 1534:111-119 Methods Mol. Biol.
    Journal
    Methods in Molecular Biology
    URI
    http://hdl.handle.net/10541/620043
    DOI
    10.1007/978-1-4939-6670-7_10
    PubMed ID
    27812872
    Type
    Article
    Language
    en
    ISSN
    1940-6029
    ae974a485f413a2113503eed53cd6c53
    10.1007/978-1-4939-6670-7_10
    Scopus Count
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    All Paterson Institute for Cancer Research

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