• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Plasma Membrane Profiling Reveals Upregulation of ABCA1 by Infected Macrophages Leading to Restriction of Mycobacterial Growth.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    fmicb-07-01086.pdf
    Size:
    1.388Mb
    Format:
    PDF
    Description:
    Open access full text article
    Download
    Authors
    Long, J
    Basu Roy, R
    Zhang, Y
    Antrobus, R
    Du, Y
    Smith, Duncan L
    Weekes, M
    Javid, B
    Affiliation
    Collaboration Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University Beijing, China
    Issue Date
    2016
    
    Metadata
    Show full item record
    Abstract
    The plasma membrane represents a critical interface between the internal and extracellular environments, and harbors multiple proteins key receptors and transporters that play important roles in restriction of intracellular infection. We applied plasma membrane profiling, a technique that combines quantitative mass spectrometry with selective cell surface aminooxy-biotinylation, to Bacille Calmette-Guérin (BCG)-infected THP-1 macrophages. We quantified 559 PM proteins in BCG-infected THP-1 cells. One significantly upregulated cell-surface protein was the cholesterol transporter ABCA1. We showed that ABCA1 was upregulated on the macrophage cell-surface following infection with pathogenic mycobacteria and knockdown of ABCA1 resulted in increased mycobacterial survival within macrophages, suggesting that it may be a novel mycobacterial host-restriction factor.
    Citation
    Plasma Membrane Profiling Reveals Upregulation of ABCA1 by Infected Macrophages Leading to Restriction of Mycobacterial Growth. 2016, 7:1086 Front Microbiol
    Journal
    Frontiers in Microbiology
    URI
    http://hdl.handle.net/10541/619929
    DOI
    10.3389/fmicb.2016.01086
    PubMed ID
    27462310
    Type
    Article
    Language
    en
    ISSN
    1664-302X
    ae974a485f413a2113503eed53cd6c53
    10.3389/fmicb.2016.01086
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • ATP-mediated killing of Mycobacterium bovis bacille Calmette-Guérin within human macrophages is calcium dependent and associated with the acidification of mycobacteria-containing phagosomes.
    • Authors: Stober CB, Lammas DA, Li CM, Kumararatne DS, Lightman SL, McArdle CA
    • Issue date: 2001 May 15
    • Comparative transcriptomics reveals common and strain-specific responses of human macrophages to infection with Mycobacterium tuberculosis and Mycobacterium bovis BCG.
    • Authors: Li P, Li Y, Wang CC, Xia LG
    • Issue date: 2024 Apr
    • The Mycobacterium bovis bacille Calmette-Guerin phagosome proteome.
    • Authors: Lee BY, Jethwaney D, Schilling B, Clemens DL, Gibson BW, Horwitz MA
    • Issue date: 2010 Jan
    • Deletion of ABCA1 and ABCG1 impairs macrophage migration because of increased Rac1 signaling.
    • Authors: Pagler TA, Wang M, Mondal M, Murphy AJ, Westerterp M, Moore KJ, Maxfield FR, Tall AR
    • Issue date: 2011 Jan 21
    • Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection.
    • Authors: Xu J, Zhou Y, Yang Y, Lv C, Liu X, Wang Y
    • Issue date: 2020 May 12
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.