Plasma Membrane Profiling Reveals Upregulation of ABCA1 by Infected Macrophages Leading to Restriction of Mycobacterial Growth.
AffiliationCollaboration Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University Beijing, China
MetadataShow full item record
AbstractThe plasma membrane represents a critical interface between the internal and extracellular environments, and harbors multiple proteins key receptors and transporters that play important roles in restriction of intracellular infection. We applied plasma membrane profiling, a technique that combines quantitative mass spectrometry with selective cell surface aminooxy-biotinylation, to Bacille Calmette-Guérin (BCG)-infected THP-1 macrophages. We quantified 559 PM proteins in BCG-infected THP-1 cells. One significantly upregulated cell-surface protein was the cholesterol transporter ABCA1. We showed that ABCA1 was upregulated on the macrophage cell-surface following infection with pathogenic mycobacteria and knockdown of ABCA1 resulted in increased mycobacterial survival within macrophages, suggesting that it may be a novel mycobacterial host-restriction factor.
CitationPlasma Membrane Profiling Reveals Upregulation of ABCA1 by Infected Macrophages Leading to Restriction of Mycobacterial Growth. 2016, 7:1086 Front Microbiol
JournalFrontiers in Microbiology
- Comprehensive insights into transcriptional adaptation of intracellular mycobacteria by microbe-enriched dual RNA sequencing.
- Authors: Rienksma RA, Suarez-Diez M, Mollenkopf HJ, Dolganov GM, Dorhoi A, Schoolnik GK, Martins Dos Santos VA, Kaufmann SH, Schaap PJ, Gengenbacher M
- Issue date: 2015 Feb 5
- The Mycobacterium bovis bacille Calmette-Guerin phagosome proteome.
- Authors: Lee BY, Jethwaney D, Schilling B, Clemens DL, Gibson BW, Horwitz MA
- Issue date: 2010 Jan
- Signaling lymphocyte-activation molecule SLAMF1 augments mycobacteria BCG-induced inflammatory response and facilitates bacterial clearance.
- Authors: Song T, Dong C, Xiong S
- Issue date: 2015 Sep
- Proteomics approach to understand reduced clearance of mycobacteria and high viral titers during HIV-mycobacteria co-infection.
- Authors: Ganji R, Dhali S, Rizvi A, Sankati S, Vemula MH, Mahajan G, Rapole S, Banerjee S
- Issue date: 2016 Mar
- [Frontier of mycobacterium research--host vs. mycobacterium].
- Authors: Okada M, Shirakawa T
- Issue date: 2005 Sep