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    Genetic profiling of tumours using both circulating free DNA and circulating tumour cells isolated from the same preserved whole blood sample.

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    Authors
    Rothwell, Dominic G
    Smith, Nigel K
    Morris, Daniel
    Leong, Hui Sun
    Li, Yaoyong
    Hollebecque, Antoine
    Ayub, Mahmood
    Carter, Louise
    Antonello, Jenny
    Franklin, Lynsey
    Miller, Crispin J
    Blackhall, Fiona H
    Dive, Caroline
    Brady, Ged
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    Affiliation
    Nucleic Acid Biomarker Laboratory, Clinical Experimental Pharmacology Group, CR-UK Manchester Institute, University of Manchester
    Issue Date
    2016-04
    
    Metadata
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    Abstract
    Molecular information obtained from cancer patients' blood is an emerging and powerful research tool with immense potential as a companion diagnostic for patient stratification and monitoring. Blood, which can be sampled routinely, provides a means of inferring the current genetic status of patients' tumours via analysis of circulating tumour cells (CTCs) or circulating tumour DNA (ctDNA). However, accurate assessment of both CTCs and ctDNA requires all blood cells to be maintained intact until samples are processed. This dictates for ctDNA analysis EDTA blood samples must be processed with 4 h of draw, severely limiting the use of ctDNA in multi-site trials. Here we describe a blood collection protocol that is amenable for analysis of both CTCs and ctDNA up to four days after blood collection. We demonstrate that yields of circulating free DNA (cfDNA) obtained from whole blood CellSave samples are equivalent to those obtained from conventional EDTA plasma processed within 4 h of blood draw. Targeted and genome-wide NGS revealed comparable DNA quality and resultant sequence information from cfDNA within CellSave and EDTA samples. We also demonstrate that CTCs and ctDNA can be isolated from the same patient blood sample, and give the same patterns of CNA enabling direct analysis of the genetic status of patients' tumours. In summary, our results demonstrate the utility of a simple approach that enabling robust molecular analysis of CTCs and cfDNA for genotype-directed therapies in multi-site clinical trials and represent a significant methodological improvement for clinical benefit.
    Citation
    Genetic profiling of tumours using both circulating free DNA and circulating tumour cells isolated from the same preserved whole blood sample. 2016, 10 (4):566-74 Mol Oncol
    Journal
    Molecular Oncology
    URI
    http://hdl.handle.net/10541/614578
    DOI
    10.1016/j.molonc.2015.11.006
    PubMed ID
    26639657
    Type
    Article
    Language
    en
    ISSN
    1878-0261
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.molonc.2015.11.006
    Scopus Count
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