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dc.contributor.authorCavazza, Tommaso
dc.contributor.authorPeset, Martin
dc.contributor.authorVernos, I
dc.date.accessioned2016-06-24T11:58:18Z
dc.date.available2016-06-24T11:58:18Z
dc.date.issued2016-05-13en
dc.identifier.citationFrom meiosis to mitosis: the sperm centrosome defines the kinetics of spindle assembly after fertilization. 2016: J Cell Scien
dc.identifier.issn1477-9137en
dc.identifier.pmid27179073en
dc.identifier.doi10.1242/jcs.183624en
dc.identifier.urihttp://hdl.handle.net/10541/614563
dc.description.abstractBipolar spindle assembly in the vertebrate oocyte relies on a self-organization chromosome-dependent pathway. Upon fertilization the male gamete provides a centrosome and the first and subsequent embryonic divisions occur in the presence of duplicated centrosomes that act as dominant microtubule organizing centres (MTOCs).The transition from meiosis to embryonic mitosis involves a necessary adaptation to integrate the dominant chromosome-dependent pathway with the centrosomes to form the bipolar spindle.Here we took advantage of the Xenopus laevis egg extract system to mimic in vitro the assembly of the first embryonic spindle and investigate the respective contribution of the centrosome and the chromosome-dependent pathway to the kinetics of the spindle bipolarization. We found that centrosomes control the transition from the meiotic to the mitotic spindle assembly mechanism. By defining the kinetics of spindle bipolarization, the centrosomes ensure their own positioning to each spindle pole and thereby their essential correct inheritance to the two first daughter cells of the embryo for the development of a healthy organism.
dc.languageENGen
dc.language.isoenen
dc.rightsArchived with thanks to Journal of cell scienceen
dc.titleFrom meiosis to mitosis: the sperm centrosome defines the kinetics of spindle assembly after fertilization.en
dc.typeArticleen
dc.contributor.departmentCell and Developmental Biology Programme, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Doctor Aiguader, 88, 08003 Barcelonaen
dc.identifier.journalJournal of Cell Scienceen
refterms.dateFOA2020-04-21T11:07:46Z
html.description.abstractBipolar spindle assembly in the vertebrate oocyte relies on a self-organization chromosome-dependent pathway. Upon fertilization the male gamete provides a centrosome and the first and subsequent embryonic divisions occur in the presence of duplicated centrosomes that act as dominant microtubule organizing centres (MTOCs).The transition from meiosis to embryonic mitosis involves a necessary adaptation to integrate the dominant chromosome-dependent pathway with the centrosomes to form the bipolar spindle.Here we took advantage of the Xenopus laevis egg extract system to mimic in vitro the assembly of the first embryonic spindle and investigate the respective contribution of the centrosome and the chromosome-dependent pathway to the kinetics of the spindle bipolarization. We found that centrosomes control the transition from the meiotic to the mitotic spindle assembly mechanism. By defining the kinetics of spindle bipolarization, the centrosomes ensure their own positioning to each spindle pole and thereby their essential correct inheritance to the two first daughter cells of the embryo for the development of a healthy organism.


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