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    From meiosis to mitosis: the sperm centrosome defines the kinetics of spindle assembly after fertilization.

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    Authors
    Cavazza, Tommaso
    Peset, Martin
    Vernos, I
    Affiliation
    Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Doctor Aiguader, 88, 08003 Barcelona
    Issue Date
    2016-05-13
    
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    Abstract
    Bipolar spindle assembly in the vertebrate oocyte relies on a self-organization chromosome-dependent pathway. Upon fertilization the male gamete provides a centrosome and the first and subsequent embryonic divisions occur in the presence of duplicated centrosomes that act as dominant microtubule organizing centres (MTOCs).The transition from meiosis to embryonic mitosis involves a necessary adaptation to integrate the dominant chromosome-dependent pathway with the centrosomes to form the bipolar spindle.Here we took advantage of the Xenopus laevis egg extract system to mimic in vitro the assembly of the first embryonic spindle and investigate the respective contribution of the centrosome and the chromosome-dependent pathway to the kinetics of the spindle bipolarization. We found that centrosomes control the transition from the meiotic to the mitotic spindle assembly mechanism. By defining the kinetics of spindle bipolarization, the centrosomes ensure their own positioning to each spindle pole and thereby their essential correct inheritance to the two first daughter cells of the embryo for the development of a healthy organism.
    Citation
    From meiosis to mitosis: the sperm centrosome defines the kinetics of spindle assembly after fertilization. 2016: J Cell Sci
    Journal
    Journal of Cell Science
    URI
    http://hdl.handle.net/10541/614563
    DOI
    10.1242/jcs.183624
    PubMed ID
    27179073
    Type
    Article
    Language
    en
    ISSN
    1477-9137
    ae974a485f413a2113503eed53cd6c53
    10.1242/jcs.183624
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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