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dc.contributor.authorHagan, Iain M
dc.date.accessioned2016-06-24T10:59:51Z
dc.date.available2016-06-24T10:59:51Z
dc.date.issued2016
dc.identifier.citationStaining fission yeast filamentous actin with fluorescent phalloidin conjugates. 2016, 2016 (6):pdb.prot091033 Cold Spring Harb Protocen
dc.identifier.issn1559-6095
dc.identifier.pmid27250943
dc.identifier.doi10.1101/pdb.prot091033
dc.identifier.urihttp://hdl.handle.net/10541/614553
dc.description.abstractThe Schizosaccharomyces pombe filamentous (F)-actin cytoskeleton drives cell growth, morphogenesis, endocytosis, and cytokinesis. The protocol described here reveals the distribution of F-actin in fixed cells through the use of fluorescently conjugated phalloidin. Simultaneous staining of cell wall landmarks (with calcofluor) and chromatin (with 4',6-diamidino-2-phenylindole, or DAPI) makes this rapid staining procedure highly effective for staging cell cycle progression, monitoring morphogenetic abnormalities, and assessing the impact of environmental and genetic changes on the integrity of the F-actin cytoskeleton.
dc.language.isoenen
dc.rightsArchived with thanks to Cold Spring Harbor protocolsen
dc.titleStaining fission yeast filamentous actin with fluorescent phalloidin conjugates.en
dc.typeArticleen
dc.contributor.departmentCRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BXen
dc.identifier.journalCold Spring Harbor Protocolsen
html.description.abstractThe Schizosaccharomyces pombe filamentous (F)-actin cytoskeleton drives cell growth, morphogenesis, endocytosis, and cytokinesis. The protocol described here reveals the distribution of F-actin in fixed cells through the use of fluorescently conjugated phalloidin. Simultaneous staining of cell wall landmarks (with calcofluor) and chromatin (with 4',6-diamidino-2-phenylindole, or DAPI) makes this rapid staining procedure highly effective for staging cell cycle progression, monitoring morphogenetic abnormalities, and assessing the impact of environmental and genetic changes on the integrity of the F-actin cytoskeleton.


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