Staining fission yeast filamentous actin with fluorescent phalloidin conjugates.
AuthorsHagan, Iain M
AffiliationCRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX
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AbstractThe Schizosaccharomyces pombe filamentous (F)-actin cytoskeleton drives cell growth, morphogenesis, endocytosis, and cytokinesis. The protocol described here reveals the distribution of F-actin in fixed cells through the use of fluorescently conjugated phalloidin. Simultaneous staining of cell wall landmarks (with calcofluor) and chromatin (with 4',6-diamidino-2-phenylindole, or DAPI) makes this rapid staining procedure highly effective for staging cell cycle progression, monitoring morphogenetic abnormalities, and assessing the impact of environmental and genetic changes on the integrity of the F-actin cytoskeleton.
CitationStaining fission yeast filamentous actin with fluorescent phalloidin conjugates. 2016, 2016 (6):pdb.prot091033 Cold Spring Harb Protoc
JournalCold Spring Harbor Protocols