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    Staining fission yeast filamentous actin with fluorescent phalloidin conjugates.

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    Authors
    Hagan, Iain M
    Affiliation
    CRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX
    Issue Date
    2016
    
    Metadata
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    Abstract
    The Schizosaccharomyces pombe filamentous (F)-actin cytoskeleton drives cell growth, morphogenesis, endocytosis, and cytokinesis. The protocol described here reveals the distribution of F-actin in fixed cells through the use of fluorescently conjugated phalloidin. Simultaneous staining of cell wall landmarks (with calcofluor) and chromatin (with 4',6-diamidino-2-phenylindole, or DAPI) makes this rapid staining procedure highly effective for staging cell cycle progression, monitoring morphogenetic abnormalities, and assessing the impact of environmental and genetic changes on the integrity of the F-actin cytoskeleton.
    Citation
    Staining fission yeast filamentous actin with fluorescent phalloidin conjugates. 2016, 2016 (6):pdb.prot091033 Cold Spring Harb Protoc
    Journal
    Cold Spring Harbor Protocols
    URI
    http://hdl.handle.net/10541/614553
    DOI
    10.1101/pdb.prot091033
    PubMed ID
    27250943
    Type
    Article
    Language
    en
    ISSN
    1559-6095
    ae974a485f413a2113503eed53cd6c53
    10.1101/pdb.prot091033
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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