Staining fission yeast filamentous actin with fluorescent phalloidin conjugates.
Authors
Hagan, Iain MAffiliation
CRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BXIssue Date
2016
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The Schizosaccharomyces pombe filamentous (F)-actin cytoskeleton drives cell growth, morphogenesis, endocytosis, and cytokinesis. The protocol described here reveals the distribution of F-actin in fixed cells through the use of fluorescently conjugated phalloidin. Simultaneous staining of cell wall landmarks (with calcofluor) and chromatin (with 4',6-diamidino-2-phenylindole, or DAPI) makes this rapid staining procedure highly effective for staging cell cycle progression, monitoring morphogenetic abnormalities, and assessing the impact of environmental and genetic changes on the integrity of the F-actin cytoskeleton.Citation
Staining fission yeast filamentous actin with fluorescent phalloidin conjugates. 2016, 2016 (6):pdb.prot091033 Cold Spring Harb ProtocJournal
Cold Spring Harbor ProtocolsDOI
10.1101/pdb.prot091033PubMed ID
27250943Type
ArticleLanguage
enISSN
1559-6095ae974a485f413a2113503eed53cd6c53
10.1101/pdb.prot091033
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