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    Cell cycle synchronization of schizosaccharomyces pombe by lactose gradient centrifugation to isolate small cells.

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    Authors
    Hagan, Iain M
    Grallert, Agnes
    Simanis, V
    Affiliation
    CRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX,
    Issue Date
    2016
    
    Metadata
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    Abstract
    Size selection of small cells from an asynchronous Schizosaccharomyces pombe culture offers a simple way to generate cultures in which progression through the mitotic cell division cycle is synchronized throughout the population. Here, we describe how density centrifugation of cells from asynchronous cultures through lactose gradients selects small G2 cells to generate synchronized cultures as large as 500 mL. The ease and simplicity of this approach makes it an accessible and attractive method for generating synchronous cultures.
    Citation
    Cell cycle synchronization of schizosaccharomyces pombe by lactose gradient centrifugation to isolate small cells. 2016, 2016 (6):pdb.prot091249 Cold Spring Harb Protoc
    Journal
    Cold Spring Harbor Protocols
    URI
    http://hdl.handle.net/10541/614552
    DOI
    10.1101/pdb.prot091249
    PubMed ID
    27250945
    Type
    Article
    Language
    en
    ISSN
    1559-6095
    ae974a485f413a2113503eed53cd6c53
    10.1101/pdb.prot091249
    Scopus Count
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    All Paterson Institute for Cancer Research

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