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dc.contributor.authorGarcia-Alegria, Eva
dc.contributor.authorIluit, M
dc.contributor.authorStefanska, Monika
dc.contributor.authorSilva, C
dc.contributor.authorHeeg, S
dc.contributor.authorKimber, S
dc.contributor.authorKouskoff, Valerie
dc.contributor.authorLacaud, Georges
dc.contributor.authorVijayaraghavan, A
dc.contributor.authorBatta, Kiran
dc.date.accessioned2016-06-24T11:09:27Z
dc.date.available2016-06-24T11:09:27Z
dc.date.issued2016en
dc.identifier.citationGraphene Oxide promotes embryonic stem cell differentiation to haematopoietic lineage. 2016, 6:25917 Sci Repen
dc.identifier.issn2045-2322en
dc.identifier.pmid27197878en
dc.identifier.doi10.1038/srep25917en
dc.identifier.urihttp://hdl.handle.net/10541/614529
dc.description.abstractPluripotent stem cells represent a promising source of differentiated tissue-specific stem and multipotent progenitor cells for regenerative medicine and drug testing. The realisation of this potential relies on the establishment of robust and reproducible protocols of differentiation. Several reports have highlighted the importance of biomaterials in assisting directed differentiation. Graphene oxide (GO) is a novel material that has attracted increasing interest in the field of biomedicine. In this study, we demonstrate that GO coated substrates significantly enhance the differentiation of mouse embryonic stem (ES) cells to both primitive and definitive haematopoietic cells. GO does not affect cell proliferation or survival of differentiated cells but rather enhances the transition of haemangioblasts to haemogenic endothelial cells, a key step during haematopoietic specification. Importantly, GO also improves, in addition to murine, human ES cell differentiation to blood cells. Taken together, our study reveals a positive role for GO in haematopoietic differentiation and suggests that further functionalization of GO could represent a valid strategy for the generation of large numbers of functional blood cells. Producing these cells would accelerate haematopoietic drug toxicity testing and treatment of patients with blood disorders or malignancies.
dc.language.isoenen
dc.rightsArchived with thanks to Scientific reportsen
dc.titleGraphene Oxide promotes embryonic stem cell differentiation to haematopoietic lineage.en
dc.typeArticleen
dc.contributor.departmentCancer Research UK Stem Cell Hematopoiesis Group, Cancer Research UK Manchester Institute, University of Manchester, Manchesteren
dc.identifier.journalScientific Reportsen
html.description.abstractPluripotent stem cells represent a promising source of differentiated tissue-specific stem and multipotent progenitor cells for regenerative medicine and drug testing. The realisation of this potential relies on the establishment of robust and reproducible protocols of differentiation. Several reports have highlighted the importance of biomaterials in assisting directed differentiation. Graphene oxide (GO) is a novel material that has attracted increasing interest in the field of biomedicine. In this study, we demonstrate that GO coated substrates significantly enhance the differentiation of mouse embryonic stem (ES) cells to both primitive and definitive haematopoietic cells. GO does not affect cell proliferation or survival of differentiated cells but rather enhances the transition of haemangioblasts to haemogenic endothelial cells, a key step during haematopoietic specification. Importantly, GO also improves, in addition to murine, human ES cell differentiation to blood cells. Taken together, our study reveals a positive role for GO in haematopoietic differentiation and suggests that further functionalization of GO could represent a valid strategy for the generation of large numbers of functional blood cells. Producing these cells would accelerate haematopoietic drug toxicity testing and treatment of patients with blood disorders or malignancies.


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