Graphene Oxide promotes embryonic stem cell differentiation to haematopoietic lineage.
Authors
Garcia-Alegria, EvaIluit, M
Stefanska, Monika
Silva, C
Heeg, S
Kimber, S
Kouskoff, Valerie
Lacaud, Georges
Vijayaraghavan, A
Batta, Kiran
Affiliation
Cancer Research UK Stem Cell Hematopoiesis Group, Cancer Research UK Manchester Institute, University of Manchester, ManchesterIssue Date
2016
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Show full item recordAbstract
Pluripotent stem cells represent a promising source of differentiated tissue-specific stem and multipotent progenitor cells for regenerative medicine and drug testing. The realisation of this potential relies on the establishment of robust and reproducible protocols of differentiation. Several reports have highlighted the importance of biomaterials in assisting directed differentiation. Graphene oxide (GO) is a novel material that has attracted increasing interest in the field of biomedicine. In this study, we demonstrate that GO coated substrates significantly enhance the differentiation of mouse embryonic stem (ES) cells to both primitive and definitive haematopoietic cells. GO does not affect cell proliferation or survival of differentiated cells but rather enhances the transition of haemangioblasts to haemogenic endothelial cells, a key step during haematopoietic specification. Importantly, GO also improves, in addition to murine, human ES cell differentiation to blood cells. Taken together, our study reveals a positive role for GO in haematopoietic differentiation and suggests that further functionalization of GO could represent a valid strategy for the generation of large numbers of functional blood cells. Producing these cells would accelerate haematopoietic drug toxicity testing and treatment of patients with blood disorders or malignancies.Citation
Graphene Oxide promotes embryonic stem cell differentiation to haematopoietic lineage. 2016, 6:25917 Sci RepJournal
Scientific ReportsDOI
10.1038/srep25917PubMed ID
27197878Type
ArticleLanguage
enISSN
2045-2322ae974a485f413a2113503eed53cd6c53
10.1038/srep25917
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