Human cytomegalovirus infection upregulates the mitochondrial transcription and translation machineries.
Authors
Karniely, SWeekes, M
Antrobus, R
Rorbach, J
van Haute, L
Umrania, Y
Smith, Duncan L
Stanton, R
Minczuk, M
Lehner, P
Sinclair, J
Affiliation
Department of Medicine, University of Cambridge Clinical School, Addenbrookes Hospital, CambridgeIssue Date
2016
Metadata
Show full item recordAbstract
Infection with human cytomegalovirus (HCMV) profoundly affects cellular metabolism. Like in tumor cells, HCMV infection increases glycolysis, and glucose carbon is shifted from the mitochondrial tricarboxylic acid cycle to the biosynthesis of fatty acids. However, unlike in many tumor cells, where aerobic glycolysis is accompanied by suppression of mitochondrial oxidative phosphorylation, HCMV induces mitochondrial biogenesis and respiration. Here, we affinity purified mitochondria and used quantitative mass spectrometry to determine how the mitochondrial proteome changes upon HCMV infection. We found that the mitochondrial transcription and translation systems are induced early during the viral replication cycle. Specifically, proteins involved in biogenesis of the mitochondrial ribosome were highly upregulated by HCMV infection. Inhibition of mitochondrial translation with chloramphenicol or knockdown of HCMV-induced ribosome biogenesis factor MRM3 abolished the HCMV-mediated increase in mitochondrially encoded proteins and significantly impaired viral growth under bioenergetically restricting conditions. Our findings demonstrate how HCMV manipulates mitochondrial biogenesis to support its replication.Citation
Human Cytomegalovirus Infection Upregulates the Mitochondrial Transcription and Translation Machineries. 2016, 7 (2): mBioJournal
mBioDOI
10.1128/mBio.00029-16PubMed ID
27025248Type
ArticleLanguage
enISSN
2150-7511ae974a485f413a2113503eed53cd6c53
10.1128/mBio.00029-16
Scopus Count
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