Fission yeast MAP kinase Sty1 is recruited to stress-induced genes.
Authors
Reiter, WolfgangWatt, Stephen
Dawson, Keren
Lawrence, Clare L
Bähler, Jürg
Jones, Nic
Wilkinson, Caroline R M
Affiliation
Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, UK.Issue Date
2008-04-11
Metadata
Show full item recordAbstract
The stress-induced expression of many fission yeast genes is dependent upon the Sty1 mitogen-activated protein kinase (MAPK) and Atf1 transcription factor. Atf1 is phosphorylated by Sty1 yet this phosphorylation is not required for stress-induced gene expression, suggesting another mechanism exists whereby Sty1 activates transcription. Here we show that Sty1 associates with Atf1-dependent genes and is recruited to both their promoters and coding regions. This occurs in response to various stress conditions coincident with the kinetics of the activation of Sty1. Association with promoters is not a consequence of increased nuclear accumulation of Sty1 nor does it require the phosphorylation of Atf1. However, recruitment is completely abolished in a mutant lacking Sty1 kinase activity. Both Atf1 and its binding partner Pcr1 are required for association of Sty1 with Atf1-dependent promoters, suggesting that this heterodimer must be intact for optimal recruitment of the MAPK. However, many Atf1-dependent genes are still expressed in a pcr1Delta mutant but with significantly delayed kinetics, thus providing an explanation for the relatively mild stress sensitivity displayed by pcr1Delta. Consistent with this delay, Sty1 and Atf1 cannot be detected at these promoters in this condition, suggesting that their association with chromatin is weak or transient in the absence of Pcr1.Citation
Fission yeast MAP kinase Sty1 is recruited to stress-induced genes. 2008, 283 (15):9945-56 J. Biol. Chem.Journal
The Journal of Biological ChemistryDOI
10.1074/jbc.M710428200PubMed ID
18252721Type
ArticleLanguage
enISSN
0021-9258ae974a485f413a2113503eed53cd6c53
10.1074/jbc.M710428200