Show simple item record

dc.contributor.authorIwasaki, Toshiyasu
dc.contributor.authorRobertson, Naomi
dc.contributor.authorTsigani, Theodora
dc.contributor.authorFinnon, Paul
dc.contributor.authorScott, David A
dc.contributor.authorLevine, Edward
dc.contributor.authorBadie, Christophe
dc.contributor.authorBouffler, Simon
dc.date.accessioned2009-04-01T23:21:34Z
dc.date.available2009-04-01T23:21:34Z
dc.date.issued2008-04
dc.identifier.citationLymphocyte telomere length correlates with in vitro radiosensitivity in breast cancer cases but is not predictive of acute normal tissue reactions to radiotherapy. 2008, 84 (4):277-84 Int. J. Radiat. Biol.en
dc.identifier.issn0955-3002
dc.identifier.pmid18386193
dc.identifier.doi10.1080/09553000801953326
dc.identifier.urihttp://hdl.handle.net/10541/58718
dc.description.abstractPURPOSE: To examine the hypothesis that lymphocyte telomere length may be predictive of both breast cancer susceptibility and severity of acute reactions to radiotherapy. MATERIALS AND METHODS: Peripheral blood lymphocyte cultures from breast cancer patients (with normal or severe skin reactions to radiotherapy) and normal individuals were assessed for in vitro radiosensitivity as measured by apoptosis, cell cycle delay and cytotoxicity. Telomere lengths were determined by a flow cytometric fluorescence in situ hybridization assay (FLOW-FISH). RESULTS: Female breast cancer cases (n = 24) had reduced lymphocyte telomere lengths by comparison with healthy controls (n = 20, p < 0.04). However, the average age of healthy controls was less (45.4) than cases (53). When the control group was modified to give a better age match (51.5, n = 13) the reduced telomere length in cases was not significantly different from controls. Lymphocytes from breast cancer cases also showed reduced cell cycle delay (p < 0.001) and increased apoptosis (p < 0.01) following irradiation in vitro at 3 and 5 Gy respectively, compared to healthy controls. Statistical significance was maintained with the improved age matching of groups. Comparison of lymphocytes from breast cancer patients with normal (n = 11) and severe (n = 13) skin reactions to radiotherapy failed to identify differences in telomere length or cellular radiosensitivity in this limited sample. CONCLUSIONS: This study adds to the evidence suggesting a correlation between altered cellular radiosensitivity and breast cancer. However, in the cases investigated, telomere length does not appear to be predictive of acute skin reactions to radiotherapy.
dc.language.isoenen
dc.subjectRadiation Sensitivityen
dc.subjectBreast Canceren
dc.subjectRadiotherapy Reactionen
dc.subjectTumour Cells
dc.subject.meshBreast Neoplasms
dc.subject.meshDose-Response Relationship, Radiation
dc.subject.meshHumans
dc.subject.meshLymphocytes
dc.subject.meshRadiation Dosage
dc.subject.meshRadiation Injuries
dc.subject.meshRadiation Tolerance
dc.subject.meshTelomere
dc.subject.meshTumor Cells, Cultured
dc.titleLymphocyte telomere length correlates with in vitro radiosensitivity in breast cancer cases but is not predictive of acute normal tissue reactions to radiotherapy.en
dc.typeArticleen
dc.contributor.departmentRadiation Effects Department, Health Protection Agency, Centre for Radiation, Chemical and Environmental Hazards, Radiation Protection Division, Chilton, Didcot, Oxfordshire, UK.en
dc.identifier.journalInternational Journal of Radiation Biologyen
html.description.abstractPURPOSE: To examine the hypothesis that lymphocyte telomere length may be predictive of both breast cancer susceptibility and severity of acute reactions to radiotherapy. MATERIALS AND METHODS: Peripheral blood lymphocyte cultures from breast cancer patients (with normal or severe skin reactions to radiotherapy) and normal individuals were assessed for in vitro radiosensitivity as measured by apoptosis, cell cycle delay and cytotoxicity. Telomere lengths were determined by a flow cytometric fluorescence in situ hybridization assay (FLOW-FISH). RESULTS: Female breast cancer cases (n = 24) had reduced lymphocyte telomere lengths by comparison with healthy controls (n = 20, p < 0.04). However, the average age of healthy controls was less (45.4) than cases (53). When the control group was modified to give a better age match (51.5, n = 13) the reduced telomere length in cases was not significantly different from controls. Lymphocytes from breast cancer cases also showed reduced cell cycle delay (p < 0.001) and increased apoptosis (p < 0.01) following irradiation in vitro at 3 and 5 Gy respectively, compared to healthy controls. Statistical significance was maintained with the improved age matching of groups. Comparison of lymphocytes from breast cancer patients with normal (n = 11) and severe (n = 13) skin reactions to radiotherapy failed to identify differences in telomere length or cellular radiosensitivity in this limited sample. CONCLUSIONS: This study adds to the evidence suggesting a correlation between altered cellular radiosensitivity and breast cancer. However, in the cases investigated, telomere length does not appear to be predictive of acute skin reactions to radiotherapy.


This item appears in the following Collection(s)

Show simple item record