The Saccharomyces cerevisiae actin cytoskeletal component Bsp1p has an auxiliary role in actomyosin ring function and in the maintenance of bud-neck structure.
AuthorsWright, Daniel J
Bentley, Adam J
Fullwood, Nigel J
Murray, Stephen M
AffiliationBiomedical Sciences Unit, Biological Sciences, Lancaster University, Lancaster LA1 4YQ, United Kingdom.
MetadataShow full item record
AbstractIqg1p is a component of the actomyosin contractile ring that is required for actin recruitment and septum deposition. Cells lacking Iqg1p function have an altered bud-neck structure and fail to form a functional actomyosin contractile ring resulting in a block to cytokinesis and septation. Here it is demonstrated that increased expression of the actin cytoskeleton associated protein Bsp1p bypasses the requirement for contractile ring function. This also correlates with reduced bud-neck width and remedial septum formation. Increased expression of this protein in a temperature-sensitive iqg1-1 background causes remedial septum formation at the bud neck that is reliant upon chitin synthase III activity and restores cell separation. The observed suppression correlates with a restoration of normal bud-neck structure. While Bsp1p is a component of the contractile ring, its recruitment to the bud neck is not required for the observed suppression. Loss of Bsp1p causes a brief delay in the redistribution of the actin cytoskeleton normally observed at the end of actin ring contraction. Compromise of Iqg1p function, in the absence of Bsp1p function, leads to a profound change in the distribution of actin and the pattern of cell growth accompanied by a failure to complete cytokinesis and cell separation.
CitationThe Saccharomyces cerevisiae actin cytoskeletal component Bsp1p has an auxiliary role in actomyosin ring function and in the maintenance of bud-neck structure. 2008, 178 (4):1903-14 Genetics
- Cdk1-dependent phosphorylation of Iqg1 governs actomyosin ring assembly prior to cytokinesis.
- Authors: Naylor SG, Morgan DO
- Issue date: 2014 Mar 1
- Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis.
- Authors: Ko N, Nishihama R, Tully GH, Ostapenko D, Solomon MJ, Morgan DO, Pringle JR
- Issue date: 2007 Dec
- The multiple roles of Cyk1p in the assembly and function of the actomyosin ring in budding yeast.
- Authors: Shannon KB, Li R
- Issue date: 1999 Feb
- IQGAP and mitotic exit network (MEN) proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae.
- Authors: Corbett M, Xiong Y, Boyne JR, Wright DJ, Munro E, Price C
- Issue date: 2006 Nov
- Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis.
- Authors: Bi E, Maddox P, Lew DJ, Salmon ED, McMillan JN, Yeh E, Pringle JR
- Issue date: 1998 Sep 7