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    The Saccharomyces cerevisiae actin cytoskeletal component Bsp1p has an auxiliary role in actomyosin ring function and in the maintenance of bud-neck structure.

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    Authors
    Wright, Daniel J
    Munro, Ewen
    Corbett, Mark
    Bentley, Adam J
    Fullwood, Nigel J
    Murray, Stephen M
    Price, Clive
    Affiliation
    Biomedical Sciences Unit, Biological Sciences, Lancaster University, Lancaster LA1 4YQ, United Kingdom.
    Issue Date
    2008-04
    
    Metadata
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    Abstract
    Iqg1p is a component of the actomyosin contractile ring that is required for actin recruitment and septum deposition. Cells lacking Iqg1p function have an altered bud-neck structure and fail to form a functional actomyosin contractile ring resulting in a block to cytokinesis and septation. Here it is demonstrated that increased expression of the actin cytoskeleton associated protein Bsp1p bypasses the requirement for contractile ring function. This also correlates with reduced bud-neck width and remedial septum formation. Increased expression of this protein in a temperature-sensitive iqg1-1 background causes remedial septum formation at the bud neck that is reliant upon chitin synthase III activity and restores cell separation. The observed suppression correlates with a restoration of normal bud-neck structure. While Bsp1p is a component of the contractile ring, its recruitment to the bud neck is not required for the observed suppression. Loss of Bsp1p causes a brief delay in the redistribution of the actin cytoskeleton normally observed at the end of actin ring contraction. Compromise of Iqg1p function, in the absence of Bsp1p function, leads to a profound change in the distribution of actin and the pattern of cell growth accompanied by a failure to complete cytokinesis and cell separation.
    Citation
    The Saccharomyces cerevisiae actin cytoskeletal component Bsp1p has an auxiliary role in actomyosin ring function and in the maintenance of bud-neck structure. 2008, 178 (4):1903-14 Genetics
    Journal
    Genetics
    URI
    http://hdl.handle.net/10541/58133
    DOI
    10.1534/genetics.107.082685
    PubMed ID
    18430924
    Type
    Article
    Language
    en
    ISSN
    0016-6731
    ae974a485f413a2113503eed53cd6c53
    10.1534/genetics.107.082685
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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