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dc.contributor.authorTang, Chi W
dc.contributor.authorMaya-Mendoza, Apolinar
dc.contributor.authorMartin, Catherine
dc.contributor.authorZeng, Kang
dc.contributor.authorChen, Songbi
dc.contributor.authorFeret, Dorota
dc.contributor.authorWilson, Stuart A
dc.contributor.authorJackson, Dean A
dc.date.accessioned2009-03-31T11:08:21Z
dc.date.available2009-03-31T11:08:21Z
dc.date.issued2008-04-01
dc.identifier.citationThe integrity of a lamin-B1-dependent nucleoskeleton is a fundamental determinant of RNA synthesis in human cells. 2008, 121 (Pt 7):1014-24 J. Cell. Sci.en
dc.identifier.issn0021-9533
dc.identifier.pmid18334554
dc.identifier.doi10.1242/jcs.020982
dc.identifier.urihttp://hdl.handle.net/10541/58113
dc.description.abstractSpatial organisation of nuclear compartments is an important regulator of chromatin function, yet the molecular principles that maintain nuclear architecture remain ill-defined. We have used RNA interference to deplete key structural nuclear proteins, the nuclear lamins. In HeLa cells, we show that reduced expression of lamin B1, but not lamin A/C, severely inhibits RNA synthesis--first by RNA polymerase II and later by RNA polymerase I. Declining levels of transcription correlate with different morphological changes in major nuclear compartments, nucleoli and nuclear speckles. Ultimately, nuclear changes linked to the loss of synthetic activity result in expansion of the inter-chromatin domain and corresponding changes in the structure and spatial organisation of chromosome territories, which relocate towards the nuclear periphery. These results show that a lamin B1-containing nucleoskeleton is required to maintain RNA synthesis and that ongoing synthesis is a fundamental determinant of global nuclear architecture in mammalian cells.
dc.language.isoenen
dc.subjectNuclear Lamin Proteinsen
dc.subjectNucleoskeletonen
dc.subjectNuclear Organisationen
dc.subjectChromosome Territoriesen
dc.subject.meshApoptosis
dc.subject.meshBlotting, Western
dc.subject.meshCell Nucleus
dc.subject.meshCells, Cultured
dc.subject.meshFluorescent Antibody Technique
dc.subject.meshHela Cells
dc.subject.meshHumans
dc.subject.meshIn Situ Hybridization, Fluorescence
dc.subject.meshLamin Type A
dc.subject.meshLamin Type B
dc.subject.meshMicroscopy, Fluorescence
dc.subject.meshNuclear Matrix
dc.subject.meshRNA
dc.subject.meshRNA Interference
dc.subject.meshRNA Polymerase I
dc.subject.meshRNA Polymerase II
dc.titleThe integrity of a lamin-B1-dependent nucleoskeleton is a fundamental determinant of RNA synthesis in human cells.en
dc.typeArticleen
dc.contributor.departmentFaculty of Life Sciences, University of Manchester, MIB, 131 Princess Street, Manchester, M1 7DN, UK.en
dc.identifier.journalJournal of Cell Scienceen
html.description.abstractSpatial organisation of nuclear compartments is an important regulator of chromatin function, yet the molecular principles that maintain nuclear architecture remain ill-defined. We have used RNA interference to deplete key structural nuclear proteins, the nuclear lamins. In HeLa cells, we show that reduced expression of lamin B1, but not lamin A/C, severely inhibits RNA synthesis--first by RNA polymerase II and later by RNA polymerase I. Declining levels of transcription correlate with different morphological changes in major nuclear compartments, nucleoli and nuclear speckles. Ultimately, nuclear changes linked to the loss of synthetic activity result in expansion of the inter-chromatin domain and corresponding changes in the structure and spatial organisation of chromosome territories, which relocate towards the nuclear periphery. These results show that a lamin B1-containing nucleoskeleton is required to maintain RNA synthesis and that ongoing synthesis is a fundamental determinant of global nuclear architecture in mammalian cells.


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