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    Characterising cytotoxic agent action as a function of the cell cycle using fourier transform infrared microspectroscopy.

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    Authors
    Jimenez-Hernandez, M
    Brown, Michael D
    Hughes, C
    Clarke, Noel W
    Gardner, P
    Affiliation
    Univ Manchester, Manchester Inst Biotechnol, Manchester 7DN UK
    Issue Date
    2015-06-15
    
    Metadata
    Show full item record
    Abstract
    Fourier Transform Infrared (FTIR) micro-spectroscopy measurements were acquired to study infrared signatures of chemotherapeutic response as a function of the cell cycle. Renal carcinoma Caki-2 cells were exposed to IC50 doses of 5-fluorouracil and Paclitaxel for a period of 24 hours. The inherent cell cycle infrared signatures from untreated and drug-treated cells were successfully retrieved by the construction of a robust SVM able to discriminate the cell cycle phases of this cell line with an average accuracy of 83.7%. The overriding infrared signature observed relates to an apoptotic biochemical response that does not appear to be correlated with the events affected by the drugs' mode of action or the cell cycle. Since apoptosis is a well conserved mechanism among living species, these results suggest that both the stages of proliferation as well as the absence/presence of apoptosis need to be taken into account in order to elucidate the fine biochemical details revealing the immediate cellular response to the drug in order to assign reliable spectral patterns of drug action.
    Citation
    Characterising cytotoxic agent action as a function of the cell cycle using fourier transform infrared microspectroscopy. 2015, 140 (13):4453-64 Analyst
    Journal
    The Analyst
    URI
    http://hdl.handle.net/10541/565775
    DOI
    10.1039/c5an00671f
    PubMed ID
    26030288
    Type
    Article
    Language
    en
    ISSN
    1364-5528
    ae974a485f413a2113503eed53cd6c53
    10.1039/c5an00671f
    Scopus Count
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    All Christie Publications

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