Show simple item record

dc.contributor.authorChen, Dongrong
dc.contributor.authorWilkinson, Caroline R M
dc.contributor.authorWatt, Stephen
dc.contributor.authorPenkett, Christopher J
dc.contributor.authorToone, W Mark
dc.contributor.authorJones, Nic
dc.contributor.authorBähler, Jürg
dc.date.accessioned2009-03-17T17:01:28Z
dc.date.available2009-03-17T17:01:28Z
dc.date.issued2008-01
dc.identifier.citationMultiple pathways differentially regulate global oxidative stress responses in fission yeast. 2008, 19 (1):308-17 Mol. Biol. Cellen
dc.identifier.issn1939-4586
dc.identifier.pmid18003976
dc.identifier.doi10.1091/mbc.E07-08-0735
dc.identifier.urihttp://hdl.handle.net/10541/56017
dc.description.abstractCellular protection against oxidative damage is relevant to ageing and numerous diseases. We analyzed the diversity of genome-wide gene expression programs and their regulation in response to various types and doses of oxidants in Schizosaccharomyces pombe. A small core gene set, regulated by the AP-1-like factor Pap1p and the two-component regulator Prr1p, was universally induced irrespective of oxidant and dose. Strong oxidative stresses led to a much larger transcriptional response. The mitogen-activated protein kinase (MAPK) Sty1p and the bZIP factor Atf1p were critical for the response to hydrogen peroxide. A newly identified zinc-finger protein, Hsr1p, is uniquely regulated by all three major regulatory systems (Sty1p-Atf1p, Pap1p, and Prr1p) and in turn globally supports gene expression in response to hydrogen peroxide. Although the overall transcriptional responses to hydrogen peroxide and t-butylhydroperoxide were similar, to our surprise, Sty1p and Atf1p were less critical for the response to the latter. Instead, another MAPK, Pmk1p, was involved in surviving this stress, although Pmk1p played only a minor role in regulating the transcriptional response. These data reveal a considerable plasticity and differential control of regulatory pathways in distinct oxidative stress conditions, providing both specificity and backup for protection from oxidative damage.
dc.language.isoenen
dc.subjectOxiative Stress Responseen
dc.subjectOxidative Damageen
dc.subjectSchizosaccharomyces Pombeen
dc.subject.meshGene Expression Regulation, Fungal
dc.subject.meshGenes, Fungal
dc.subject.meshHydrogen Peroxide
dc.subject.meshMitogen-Activated Protein Kinase Kinases
dc.subject.meshOxidants
dc.subject.meshOxidative Stress
dc.subject.meshSchizosaccharomyces
dc.subject.meshSchizosaccharomyces Pombe Proteins
dc.subject.meshSignal Transduction
dc.subject.meshVitamin K 3
dc.subject.meshtert-Butylhydroperoxide
dc.titleMultiple pathways differentially regulate global oxidative stress responses in fission yeast.en
dc.typeArticleen
dc.contributor.departmentCancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, United Kingdom.en
dc.identifier.journalMolecular Biology of the Cellen
html.description.abstractCellular protection against oxidative damage is relevant to ageing and numerous diseases. We analyzed the diversity of genome-wide gene expression programs and their regulation in response to various types and doses of oxidants in Schizosaccharomyces pombe. A small core gene set, regulated by the AP-1-like factor Pap1p and the two-component regulator Prr1p, was universally induced irrespective of oxidant and dose. Strong oxidative stresses led to a much larger transcriptional response. The mitogen-activated protein kinase (MAPK) Sty1p and the bZIP factor Atf1p were critical for the response to hydrogen peroxide. A newly identified zinc-finger protein, Hsr1p, is uniquely regulated by all three major regulatory systems (Sty1p-Atf1p, Pap1p, and Prr1p) and in turn globally supports gene expression in response to hydrogen peroxide. Although the overall transcriptional responses to hydrogen peroxide and t-butylhydroperoxide were similar, to our surprise, Sty1p and Atf1p were less critical for the response to the latter. Instead, another MAPK, Pmk1p, was involved in surviving this stress, although Pmk1p played only a minor role in regulating the transcriptional response. These data reveal a considerable plasticity and differential control of regulatory pathways in distinct oxidative stress conditions, providing both specificity and backup for protection from oxidative damage.


This item appears in the following Collection(s)

Show simple item record