Int6/eIF3e promotes general translation and Atf1 abundance to modulate Sty1 MAPK-dependent stress response in fission yeast.
Wilkinson, Caroline R M
AffiliationMolecular Cellular and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, Kansas 66506, USA.
MetadataShow full item record
Abstractint-6 is one of the frequent integration sites for mouse mammary tumor viruses. Although its product is the e-subunit of translation initiation factor eIF3, other evidence indicates that it interacts with proteasomes or other proteins to regulate protein stability. Here we report that the fission yeast int6(+) is required for overcoming stress imposed by histidine starvation, using the drug 3-aminotriazole (3AT). Microarray and complementary Northern studies using wild-type, int6Delta or gcn2Delta mutants indicate that 3AT-treated wild-type yeast induces core environmental stress response (CESR) genes in addition to typical general amino acid control (GAAC) genes whose transcription depends on the eIF2 kinase, Gcn2. In agreement with this, Sty1 MAPK and its target transcription factor Atf1, which signal the CESR, are required for overcoming 3AT-induced starvation. We find that Int6 is required for maintaining the basal level of Atf1 and for rapid transcriptional activation of the CESR on 3AT-insult. Pulse labeling experiments indicate that int6Delta significantly slows down de novo protein synthesis. Moreover, Atf1 protein half-life was reduced in int6Delta cells. These effects would account for the compromised Atf1 activity on 3AT-induced stress. Thus, the robust protein synthesis promoted by intact eIF3 appears to be a part of the requisites for sound Sty1 MAPK-dependent signaling governed by the activity of the Atf1 transcription factor.
CitationInt6/eIF3e promotes general translation and Atf1 abundance to modulate Sty1 MAPK-dependent stress response in fission yeast. 2008, 283 (32):22063-75 J. Biol. Chem.
JournalThe Journal of Biological Chemistry
- The roles of stress-activated Sty1 and Gcn2 kinases and of the protooncoprotein homologue Int6/eIF3e in responses to endogenous oxidative stress during histidine starvation.
- Authors: Nemoto N, Udagawa T, Ohira T, Jiang L, Hirota K, Wilkinson CR, Bähler J, Jones N, Ohta K, Wek RC, Asano K
- Issue date: 2010 Nov 26
- Deciphering the role of the signal- and Sty1 kinase-dependent phosphorylation of the stress-responsive transcription factor Atf1 on gene activation.
- Authors: Salat-Canela C, Paulo E, Sánchez-Mir L, Carmona M, Ayté J, Oliva B, Hidalgo E
- Issue date: 2017 Aug 18
- Fission yeast mitogen-activated protein kinase Sty1 interacts with translation factors.
- Authors: Asp E, Nilsson D, Sunnerhagen P
- Issue date: 2008 Feb
- Genomic binding profiling of the fission yeast stress-activated MAPK Sty1 and the bZIP transcriptional activator Atf1 in response to H2O2.
- Authors: Eshaghi M, Lee JH, Zhu L, Poon SY, Li J, Cho KH, Chu Z, Karuturi RK, Liu J
- Issue date: 2010 Jul 16
- The Atf1 transcription factor is a target for the Sty1 stress-activated MAP kinase pathway in fission yeast.
- Authors: Wilkinson MG, Samuels M, Takeda T, Toone WM, Shieh JC, Toda T, Millar JB, Jones N
- Issue date: 1996 Sep 15