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    The Basal Transcription Complex Component TAF3 Transduces Changes in Nuclear Phosphoinositides into Transcriptional Output.

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    Authors
    Stijf-Bultsma, Yvette
    Sommer, Lilly
    Tauber, M
    Baalbaki, M
    Giardoglou, P
    Jones, D
    Gelato, K
    van Pelt, J
    Shah, Z
    Rahnamoun, H
    Toma, C
    Anderson, K
    Hawkins, P
    Lauberth, S
    Haramis, A
    Hart, D
    Fischle, W
    Divecha, N
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    Affiliation
    The Inositide Laboratory, Centre for Biological Sciences, Highfield Campus, University of Southampton, Southampton
    Issue Date
    2015-05-07
    
    Metadata
    Show full item record
    Abstract
    Phosphoinositides (PI) are important signaling molecules in the nucleus that influence gene expression. However, if and how nuclear PI directly affects the transcriptional machinery is not known. We report that the lipid kinase PIP4K2B regulates nuclear PI5P and the expression of myogenic genes during myoblast differentiation. A targeted screen for PI interactors identified the PHD finger of TAF3, a TATA box binding protein-associated factor with important roles in transcription regulation, pluripotency, and differentiation. We show that the PI interaction site is distinct from the known H3K4me3 binding region of TAF3 and that PI binding modulates association of TAF3 with H3K4me3 in vitro and with chromatin in vivo. Analysis of TAF3 mutants indicates that TAF3 transduces PIP4K2B-mediated alterations in PI into changes in specific gene transcription. Our study reveals TAF3 as a direct target of nuclear PI and further illustrates the importance of basal transcription components as signal transducers.
    Citation
    The Basal Transcription Complex Component TAF3 Transduces Changes in Nuclear Phosphoinositides into Transcriptional Output. 2015, 58 (3):453-67 Mol Cell
    Journal
    Molecular Cell
    URI
    http://hdl.handle.net/10541/559299
    DOI
    10.1016/j.molcel.2015.03.009
    PubMed ID
    25866244
    Type
    Article
    Language
    en
    ISSN
    1097-4164
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.molcel.2015.03.009
    Scopus Count
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