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    Enhanced FTIR bench-top imaging of single biological cells.

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    Authors
    Hughes, C
    Henderson, A
    Kansiz, M
    Dorling, K
    Jimenez-Hernandez, M
    Brown, Michael D
    Clarke, Noel W
    Gardner, P
    Affiliation
    Manchester Institute of Biotechnology, The University of Manchester, 131 Princess Street, Manchester
    Issue Date
    2015-03-16
    
    Metadata
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    Abstract
    A new optical system has recently been developed that enables infrared images to be obtained with a pixel resolution of 1 micron on a bench-top instrument using a thermal source. We present here imaging data from two contrasting cellular systems that represent different challenges. Renal carcinoma cells cytospun onto CaF2 have a largely rounded morphology and thus suffer from strong resonant Mie scattering. Skin fibroblast cells, cultured onto CaF2 on the other hand are very spread out so scatter less strongly but are so thin they deliver extremely weak signals. Using suitable pre-processing methods, including PCA noise reduction and RMieS correction, we demonstrate that useful high resolution images can be obtained from either sample.
    Citation
    Enhanced FTIR bench-top imaging of single biological cells. 2015, 140 (7):2080-5 Analyst
    Journal
    The Analyst
    URI
    http://hdl.handle.net/10541/550188
    DOI
    10.1039/c4an02053g
    PubMed ID
    25738183
    Type
    Article
    Language
    en
    ISSN
    1364-5528
    ae974a485f413a2113503eed53cd6c53
    10.1039/c4an02053g
    Scopus Count
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