Authors
Hughes, CHenderson, A
Kansiz, M
Dorling, K
Jimenez-Hernandez, M
Brown, Michael D
Clarke, Noel W
Gardner, P
Affiliation
Manchester Institute of Biotechnology, The University of Manchester, 131 Princess Street, ManchesterIssue Date
2015-03-16
Metadata
Show full item recordAbstract
A new optical system has recently been developed that enables infrared images to be obtained with a pixel resolution of 1 micron on a bench-top instrument using a thermal source. We present here imaging data from two contrasting cellular systems that represent different challenges. Renal carcinoma cells cytospun onto CaF2 have a largely rounded morphology and thus suffer from strong resonant Mie scattering. Skin fibroblast cells, cultured onto CaF2 on the other hand are very spread out so scatter less strongly but are so thin they deliver extremely weak signals. Using suitable pre-processing methods, including PCA noise reduction and RMieS correction, we demonstrate that useful high resolution images can be obtained from either sample.Citation
Enhanced FTIR bench-top imaging of single biological cells. 2015, 140 (7):2080-5 AnalystJournal
The AnalystDOI
10.1039/c4an02053gPubMed ID
25738183Type
ArticleLanguage
enISSN
1364-5528ae974a485f413a2113503eed53cd6c53
10.1039/c4an02053g
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