Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP).
Authors
El Bounkari, OmarGuria, Anuja
Klebba-Faerber, Sabine
Claussen, Maike
Pieler, Tomas
Griffiths, John R
Whetton, Anthony D
Koch, Alexandra
Tamura, Teruko
Affiliation
Institut fuer Biochemie, OE4310 Medizinische Hochschule Hannover, Hannover, Germany.Issue Date
2009-01-05
Metadata
Show full item recordAbstract
THOC7 and Fms-interacting protein (FMIP) are members of the THO complex that associate with the mRNA export apparatus. FMIP is a nucleocytoplasmic shuttling protein with a nuclear localization signal (NLS), whereas THOC7 does not contain a typical NLS motif. We show here that THOC7 (50-137, amino acid numbers) binds to the N-terminal portion (1-199) of FMIP directly. FMIP is detected mainly in the nucleus. In the absence of exogenous FMIP, THOC7 resides mainly in the cytoplasm, while in the presence of FMIP, THOC7 is transported into the nucleus with FMIP. Furthermore, THOC7 lacking the FMIP binding site does not co-localize with FMIP, indicating that THOC7/FMIP interaction is required for nuclear localization of THOC7.Citation
Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP). 2009, 583 (1):13-8 FEBS Lett.Journal
FEBS LettersDOI
10.1016/j.febslet.2008.11.024PubMed ID
19059247Type
ArticleLanguage
enISSN
1873-3468ae974a485f413a2113503eed53cd6c53
10.1016/j.febslet.2008.11.024
Scopus Count
Related articles
- FMIP controls the adipocyte lineage commitment of C2C12 cells by downmodulation of C/EBP alpha.
- Authors: Mancini A, El Bounkari O, Norrenbrock AF, Scherr M, Schaefer D, Eder M, Banham AH, Pulford K, Lyne L, Whetton AD, Tamura T
- Issue date: 2007 Feb 15
- The M-CSF receptor substrate and interacting protein FMIP is governed in its subcellular localization by protein kinase C-mediated phosphorylation, and thereby potentiates M-CSF-mediated differentiation.
- Authors: Mancini A, Koch A, Whetton AD, Tamura T
- Issue date: 2004 Aug 26
- RNA-binding of the human cytomegalovirus transactivator protein UL69, mediated by arginine-rich motifs, is not required for nuclear export of unspliced RNA.
- Authors: Toth Z, Lischka P, Stamminger T
- Issue date: 2006
- The redox state of SECIS binding protein 2 controls its localization and selenocysteine incorporation function.
- Authors: Papp LV, Lu J, Striebel F, Kennedy D, Holmgren A, Khanna KK
- Issue date: 2006 Jul
- Analysis of a nuclear localization signal in the p14 splicing factor in Trypanosoma cruzi.
- Authors: Westergaard GG, Bercovich N, Reinert MD, Vazquez MP
- Issue date: 2010 Aug 1