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dc.contributor.authorRothwell, Dominic G
dc.contributor.authorLi, Yaoyong
dc.contributor.authorAyub, Mahmood
dc.contributor.authorTate, Catriona
dc.contributor.authorNewton, Gillian
dc.contributor.authorHey, Yvonne
dc.contributor.authorCarter, Louise
dc.contributor.authorFaulkner, Suzanne
dc.contributor.authorMoro, Massimo
dc.contributor.authorPepper, Stuart D
dc.contributor.authorMiller, Crispin J
dc.contributor.authorBlackhall, Fiona H
dc.contributor.authorBertolini, G
dc.contributor.authorRoz, L
dc.contributor.authorDive, Caroline
dc.contributor.authorBrady, Ged
dc.date.accessioned2015-03-19T11:33:46Zen
dc.date.available2015-03-19T11:33:46Zen
dc.date.issued2014en
dc.identifier.citationEvaluation and validation of a robust single cell RNA-amplification protocol through transcriptional profiling of enriched lung cancer initiating cells. 2014, 15:1129 BMC Genomicsen
dc.identifier.issn1471-2164en
dc.identifier.pmid25519510en
dc.identifier.doi10.1186/1471-2164-15-1129en
dc.identifier.urihttp://hdl.handle.net/10541/346867en
dc.description.abstractAlthough profiling of RNA in single cells has broadened our understanding of development, cancer biology and mechanisms of disease dissemination, it requires the development of reliable and flexible methods. Here we demonstrate that the EpiStem RNA-Amp™ methodology reproducibly generates microgram amounts of cDNA suitable for RNA-Seq, RT-qPCR arrays and Microarray analysis.
dc.language.isoenen
dc.rightsArchived with thanks to BMC genomicsen
dc.titleEvaluation and validation of a robust single cell RNA-amplification protocol through transcriptional profiling of enriched lung cancer initiating cells.en
dc.typeArticleen
dc.contributor.department"Nucleic Acid Biomarker Laboratory, Clinical & Experimental Pharmacology, CR-UK Manchester Institute, University of Manchester, Manchester M20 4BX, UK.en
dc.identifier.journalBMC Genomicsen
html.description.abstractAlthough profiling of RNA in single cells has broadened our understanding of development, cancer biology and mechanisms of disease dissemination, it requires the development of reliable and flexible methods. Here we demonstrate that the EpiStem RNA-Amp™ methodology reproducibly generates microgram amounts of cDNA suitable for RNA-Seq, RT-qPCR arrays and Microarray analysis.


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