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    Molecular phenotyping of a UK population: defining the human serum metabolome.

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    Authors
    Dunn, W
    Lin, W
    Broadhurst, D
    Begley, P
    Brown, M
    Zelena, E
    Vaughan, A
    Halsall, A
    Harding, N
    Knowles, J
    Francis-McIntyre, S
    Tseng, A
    Ellis, D
    O'Hagan, S
    Aarons, G
    Benjamin, B
    Chew-Graham, S
    Moseley, Carly
    Potter, Paula
    Winder, C
    Potts, C
    Thornton, P
    McWhirter, C
    Zubair, M
    Pan, M
    Burns, A
    Cruickshank, J
    Jayson, Gordon C
    Purandare, N
    Wu, Frederick C W
    Finn, J
    Haselden, J
    Nicholls, A
    Wilson, I
    Goodacre, R
    Kell, D
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    Affiliation
    Faculty of Engineering and Physical Sciences, School of Chemistry, Manchester Institute of Biotechnology, The University of Manchester, Manchester, M1 7DN UK
    Issue Date
    2015
    
    Metadata
    Show full item record
    Abstract
    Phenotyping of 1,200 'healthy' adults from the UK has been performed through the investigation of diverse classes of hydrophilic and lipophilic metabolites present in serum by applying a series of chromatography-mass spectrometry platforms. These data were made robust to instrumental drift by numerical correction; this was prerequisite to allow detection of subtle metabolic differences. The variation in observed metabolite relative concentrations between the 1,200 subjects ranged from less than 5 % to more than 200 %. Variations in metabolites could be related to differences in gender, age, BMI, blood pressure, and smoking. Investigations suggest that a sample size of 600 subjects is both necessary and sufficient for robust analysis of these data. Overall, this is a large scale and non-targeted chromatographic MS-based metabolomics study, using samples from over 1,000 individuals, to provide a comprehensive measurement of their serum metabolomes. This work provides an important baseline or reference dataset for understanding the 'normal' relative concentrations and variation in the human serum metabolome. These may be related to our increasing knowledge of the human metabolic network map. Information on the Husermet study is available at http://www.husermet.org/. Importantly, all of the data are made freely available at MetaboLights (http://www.ebi.ac.uk/metabolights/).
    Citation
    Molecular phenotyping of a UK population: defining the human serum metabolome., 11:9-26 Metabolomics
    Journal
    Metabolomics
    URI
    http://hdl.handle.net/10541/345316
    DOI
    10.1007/s11306-014-0707-1
    PubMed ID
    25598764
    Type
    Article
    Language
    en
    ISSN
    1573-3882
    ae974a485f413a2113503eed53cd6c53
    10.1007/s11306-014-0707-1
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