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dc.contributor.authorMiller, G
dc.contributor.authorHansen, S
dc.contributor.authorBaráth, M
dc.contributor.authorJohannessen, C
dc.contributor.authorBlanch, E
dc.contributor.authorJayson, Gordon C
dc.contributor.authorGardiner, J
dc.date.accessioned2015-01-08T11:10:36Z
dc.date.available2015-01-08T11:10:36Z
dc.date.issued2014-12-05
dc.identifier.citationSynthesis of a heparin-related GlcN-IdoA sulfation-site variable disaccharide library and analysis by Raman and ROA spectroscopy. 2014, 400:44-53 Carbohydr Resen
dc.identifier.issn1873-426X
dc.identifier.pmid25457609
dc.identifier.doi10.1016/j.carres.2014.06.026
dc.identifier.urihttp://hdl.handle.net/10541/337936
dc.description.abstractSynthesis of an array of differentially sulfated GlcN-IdoA disaccharides, accessible on good scale, directly from l-iduronate components is described. These are specifically directed to provide the sulfation variability at the key most common biologically relevant sulfation-variable l-IdoA O-2 and d-GlcN O-6 and amino sites of this heparin disaccharide. This sulfation-varied matrix has allowed the first evaluation of using Raman/ROA spectroscopy to characterize changes in spectra as a function of both site and level of sulfation with pure, defined heparin-related disaccharide species. This provides analysis of both similarities and differences to digest native heparin and this shows evidence of different types of changes in conformations and conformational freedom as a function of some specific sulfation changes at the disaccharide level. It is anticipated that this data set will open the way for applications to further site-specific sulfated saccharides and demonstrates the capability offered by Raman-ROA towards fingerprinting sulfation in heparin fragments.
dc.language.isoenen
dc.rightsArchived with thanks to Carbohydrate researchen
dc.titleSynthesis of a heparin-related GlcN-IdoA sulfation-site variable disaccharide library and analysis by Raman and ROA spectroscopy.en
dc.typeArticleen
dc.contributor.departmentManchester Institute of Biotechnology and School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DNen
dc.identifier.journalCarbohydrate Researchen
html.description.abstractSynthesis of an array of differentially sulfated GlcN-IdoA disaccharides, accessible on good scale, directly from l-iduronate components is described. These are specifically directed to provide the sulfation variability at the key most common biologically relevant sulfation-variable l-IdoA O-2 and d-GlcN O-6 and amino sites of this heparin disaccharide. This sulfation-varied matrix has allowed the first evaluation of using Raman/ROA spectroscopy to characterize changes in spectra as a function of both site and level of sulfation with pure, defined heparin-related disaccharide species. This provides analysis of both similarities and differences to digest native heparin and this shows evidence of different types of changes in conformations and conformational freedom as a function of some specific sulfation changes at the disaccharide level. It is anticipated that this data set will open the way for applications to further site-specific sulfated saccharides and demonstrates the capability offered by Raman-ROA towards fingerprinting sulfation in heparin fragments.


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